Samantha Tamrakar1, Masakazu Yashiro2,3, Toshiyuki Kawashima1, Takehiro Uda1, Yuzo Terakawa1, Yuko Kuwae4, Masahiko Ohsawa4, Kenji Ohata1. 1. Department of Neurosurgery, Osaka City University Graduate School of Medicine, Osaka, Japan. 2. Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan m9312510@med.osaka-cu.ac.jp. 3. Molecular Oncology and Therapeutics, Osaka City University Graduate School of Medicine, Osaka, Japan. 4. Department of Diagnostic Pathology and Molecular Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan.
Abstract
AIM: To investigate clinicopathological significance of autophagy and its association with genetic alterations in gliomas. MATERIALS AND METHODS: The expression of three autophagy-related proteins, light chain-3 (LC3), beclin 1, and p62 was immunohistochemically analyzed in 32 low-grade gliomas and 65 high-grade gliomas. RESULTS: LC3, beclin 1, and p62 expression was positive in 70/94 (74%), 51/94 (54%) and 55/96 (57%) gliomas, respectively. High expression of LC3, beclin 1 and p62 was significantly more frequent in high-grade gliomas than in low-grade. Positive expression of LC3, beclin 1 and p62 were significantly positively correlated with overall survival, methylation of O6-methylyguanine-DNA methyltransferase (MGMT) promoter, mutations of isocitrate dehydrogenase 1 (IDH1) and telomerase reverse transcriptase (TERT) promoter, and 1p/19q co-deletion. Kaplan-Meier analyses revealed that LC3, p62 and autophagy status (positivity for at least two of the three proteins) were significantly associated with poorer survival. CONCLUSION: Autophagy might be associated with the progression of glioma, particularly high-grade, and thus might be a useful prognostic factor in patients with glioma. Copyright
AIM: To investigate clinicopathological significance of autophagy and its association with genetic alterations in gliomas. MATERIALS AND METHODS: The expression of three autophagy-related proteins, light chain-3 (LC3), beclin 1, and p62 was immunohistochemically analyzed in 32 low-grade gliomas and 65 high-grade gliomas. RESULTS:LC3, beclin 1, and p62 expression was positive in 70/94 (74%), 51/94 (54%) and 55/96 (57%) gliomas, respectively. High expression of LC3, beclin 1 and p62 was significantly more frequent in high-grade gliomas than in low-grade. Positive expression of LC3, beclin 1 and p62 were significantly positively correlated with overall survival, methylation of O6-methylyguanine-DNA methyltransferase (MGMT) promoter, mutations of isocitrate dehydrogenase 1 (IDH1) and telomerase reverse transcriptase (TERT) promoter, and 1p/19q co-deletion. Kaplan-Meier analyses revealed that LC3, p62 and autophagy status (positivity for at least two of the three proteins) were significantly associated with poorer survival. CONCLUSION: Autophagy might be associated with the progression of glioma, particularly high-grade, and thus might be a useful prognostic factor in patients with glioma. Copyright
Authors: Alejandro Urdiciain; Elena Erausquin; María V Zelaya; Idoya Zazpe; José L Lanciego; Bárbara Meléndez; Juan A Rey; Miguel A Idoate; Natalia A Riobo-Del Galdo; Javier S Castresana Journal: Biology (Basel) Date: 2021-05-26