| Literature DB >> 30841101 |
Chenggui Han1, Dawei Li1, Yiming Xing1, Kun Zhu1, Zhaofeng Tian1, Zhunan Cai1, Jialin Yu1, Yi Liu1.
Abstract
Wheat yellow mosaic virus (WYMV) was identified in 25 of 27 wheat (Triticum aestivum) samples from six provinces in China using reverse-transcription polymerase chain reaction. Wheat spindle streak mosaic virus was not detected. Thus, contrary to previous reports, WYMV was the only bymovirus detected in wheat in China. The cDNA fragments corresponding to coat protein coding regions of five WYMV isolates from China were cloned and sequenced. Deduced amino acid comparison of coat proteins among these five isolates, another Chinese WYMV isolate HC, and one isolate from Japan showed very high sequence conservation, particularly in both the N- and C- termini. However, a highly virulent isolate HC from Henan Province showed four amino acid substitutions in highly conserved amino acid positions which might play a role in its pathogenic variation.Entities:
Keywords: immunosorbent electron microscopy
Year: 2000 PMID: 30841101 DOI: 10.1094/PDIS.2000.84.6.627
Source DB: PubMed Journal: Plant Dis ISSN: 0191-2917 Impact factor: 4.438