Mehmet Sürmeli1, Salih Maçin2, Yakut Akyön3, Aycan Uğur Kayikçioğlu4. 1. Plastic, Reconstructive and Aesthetic Surgery Specialist, Istanbul Bağcilar Research and Training Hospital, Merkez Mah, Department of Plastic, Reconstructive and Aesthetic Surgery, 34200 Bağcilar, Istanbul, Turkey. 2. Microbiology and Medical Microbiology Specialist, Selçuk University Faculty of Medicine Departmant of Medical Microbiology, Konya, Turkey. 3. Professor of Microbiology and Medical Microbiology, Hacettepe University Faculty of Medicine, Sihhiye Ankara, Turkey. 4. Professor of Plastic, Reconstructive and Aesthetic Surgery, Hacettepe University Faculty of Medicine, Sihhiye Ankara, Turkey.
Abstract
OBJECTIVE: To determine and quantitatively measure the protective and/or therapeutic effect of Lactobacillus plantarum (LP) application on a burn wound before and after the onset of meticillin-resistant Staphylococcus aureus (MRSA) infection. METHOD: Third-degree scald burns affecting 10% of body surface area were formed on the back of five groups of rats. Group 1 was designated as the control group. In Group 2, LP was applied immediately after the burn and then MRSA inoculated. In Group 3, MRSA was applied immediately after the burn and then LP inoculated. Groups 4 and 5 were designated as controls of LP and MRSA. On the fifth and tenth days, bacterial loads and compositions were assessed by tissue biopsies. RESULTS: Each group contained seven rats (n=35). In Group 2, MRSA colony counts were found to be significantly lower compared with the other groups (p<0.05). In Group 3, MRSA colony counts were not found to be significantly different compared with control groups (p>0.05). CONCLUSION: LP was shown to have a protective role in non-infected, burn wounds when applied before MRSA infection but a therapeutic effect of LP was not demonstrated. LP is thought to have a promising role in the prevention and treatment of burn wound infections.
OBJECTIVE: To determine and quantitatively measure the protective and/or therapeutic effect of Lactobacillus plantarum (LP) application on a burn wound before and after the onset of meticillin-resistant Staphylococcus aureus (MRSA) infection. METHOD: Third-degree scald burns affecting 10% of body surface area were formed on the back of five groups of rats. Group 1 was designated as the control group. In Group 2, LP was applied immediately after the burn and then MRSA inoculated. In Group 3, MRSA was applied immediately after the burn and then LP inoculated. Groups 4 and 5 were designated as controls of LP and MRSA. On the fifth and tenth days, bacterial loads and compositions were assessed by tissue biopsies. RESULTS: Each group contained seven rats (n=35). In Group 2, MRSA colony counts were found to be significantly lower compared with the other groups (p<0.05). In Group 3, MRSA colony counts were not found to be significantly different compared with control groups (p>0.05). CONCLUSION:LP was shown to have a protective role in non-infected, burn wounds when applied before MRSA infection but a therapeutic effect of LP was not demonstrated. LP is thought to have a promising role in the prevention and treatment of burn wound infections.
Authors: Sabina Fijan; Anita Frauwallner; Tomaž Langerholc; Bojan Krebs; Jessica A Ter Haar Née Younes; Adolf Heschl; Dušanka Mičetić Turk; Irena Rogelj Journal: Biomed Res Int Date: 2019-12-12 Impact factor: 3.411