Y Jin1, Z-L Zhang, Y Huang, K-N Zhang, B Xiong. 1. Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China. binxiong1961@163.com.
Abstract
OBJECTIVE: To investigate the role and molecular mechanism of miR-182-5p in the development of colorectal cancer (CRC), thereby providing a theoretical basis for new CRC therapeutic targets. PATIENTS AND METHODS: The expression of miR-182-5p in CRC clinical cases and biological cell lines was detected. On-line target gene prediction and Luciferase reporter gene assay were performed to screen and verify the target of miR-182-5p, respectively. The role of miR-182-5p in CRC cell function was further analyzed. RESULTS: MiR-182-5p expression was significantly decreased in both CRC tissues and cell lines. Metadherin (MTDH) was screened and verified as a functional target of miR-182-5p. The cell proliferation, invasion and migration ability of CRC cells were significantly inhibited after the up-regulation of miR-182-5p. However, MTDH limited the anti-cancer effects of miR-182-5p in CRC cells. CONCLUSIONS: Our research demonstrated the inhibitory function of miR-182-5p in CRC. Therefore, the miR-182-5p/MTDH axis was expected to be one of the targets of CRC targeted therapy.
OBJECTIVE: To investigate the role and molecular mechanism of miR-182-5p in the development of colorectal cancer (CRC), thereby providing a theoretical basis for new CRC therapeutic targets. PATIENTS AND METHODS: The expression of miR-182-5p in CRC clinical cases and biological cell lines was detected. On-line target gene prediction and Luciferase reporter gene assay were performed to screen and verify the target of miR-182-5p, respectively. The role of miR-182-5p in CRC cell function was further analyzed. RESULTS:MiR-182-5p expression was significantly decreased in both CRC tissues and cell lines. Metadherin (MTDH) was screened and verified as a functional target of miR-182-5p. The cell proliferation, invasion and migration ability of CRC cells were significantly inhibited after the up-regulation of miR-182-5p. However, MTDH limited the anti-cancer effects of miR-182-5p in CRC cells. CONCLUSIONS: Our research demonstrated the inhibitory function of miR-182-5p in CRC. Therefore, the miR-182-5p/MTDH axis was expected to be one of the targets of CRC targeted therapy.
Authors: Jianling Bi; Shujie Yang; Long Li; Qun Dai; Nicholas Borcherding; Brett A Wagner; Garry R Buettner; Douglas R Spitz; Kimberly K Leslie; Jun Zhang; Xiangbing Meng Journal: Cell Death Dis Date: 2019-09-17 Impact factor: 8.469