| Literature DB >> 30840234 |
Shasha Yao1, Yifei Xu2,3, Changyu Shao1, Fabio Nudelman4, Nico A J M Sommerdijk2,3, Ruikang Tang5.
Abstract
The bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is self-assembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimensional (2D) and three-dimensional (3D) stochastic optical reconstruction microscopy (STORM), which enables in situ and high-resolution visualization of the process.Entities:
Keywords: Bone; Calcium phosphate; CryoTEM; STORM; Type-I collagen
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Year: 2019 PMID： 30840234 DOI： 10.1007/978-1-4939-9095-5_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745