Literature DB >> 30839251

Whole-genome-based analysis reveals multiclone Serratia marcescens outbreaks in a non-Neonatal Intensive Care Unit setting in a tertiary care hospital in India.

Anusha Rohit1, D Suresh Kumar1, I Dhinakaran1, Jessy Joy1, Deekshit Vijay Kumar2, Krishna Kumar Ballamoole2, I Karunasagar2, Prior Karola3, Harmsen Dag3.   

Abstract

We report the use of next generation sequencing (NGS) for investigating an outbreak of 13 cases of Serratia marcescens blood stream infections in a non-Neonatal Intensive Care Unit (non-NICU) setting in a tertiary care hospital in India over 5 months. Thirteen cases of sepsis due to S. marcescens were identified in various Intensive Care Units (ICUs) over 5 months. Environmental surveillance identified isolates in the adult ICU (AICU). Antibiogram did not correlate with timeline. Sequencing libraries were prepared using Nextera XT chemistry (Illumina). Based on NGS, two clusters were identified. Cluster 1 had environmental and clinical isolates from the AICU and cluster 2 were isolates from the Coronary Care Unit (CCU). NICU and Paediatric ICU isolates did not belong to any cluster. Polyclonal outbreaks best identified by NGS can occur simultaneously. Good infection prevention practices like hand hygiene for compounded medicines and surface cleaning helped end the outbreak.

Entities:  

Keywords:  next generation sequencing; non-NICU Serratia marcescens; outbreak investigation

Mesh：

Year:  2019        PMID： 30839251     DOI： 10.1099/jmm.0.000947

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  5 in total

Review 1.  Third-Generation Sequencing in the Clinical Laboratory: Exploring the Advantages and Challenges of Nanopore Sequencing.

Authors:  Lauren M Petersen; Isabella W Martin; Wayne E Moschetti; Colleen M Kershaw; Gregory J Tsongalis
Journal:  J Clin Microbiol       Date:  2019-12-23       Impact factor: 5.948

2.  Characterization of antimicrobial-resistant Gram-negative bacteria that cause neonatal sepsis in seven low- and middle-income countries.

Authors:  Kirsty Sands; Maria J Carvalho; Edward Portal; Kathryn Thomson; Calie Dyer; Chinenye Akpulu; Robert Andrews; Ana Ferreira; David Gillespie; Thomas Hender; Kerenza Hood; Jordan Mathias; Rebecca Milton; Maria Nieto; Khadijeh Taiyari; Grace J Chan; Delayehu Bekele; Semaria Solomon; Sulagna Basu; Pinaki Chattopadhyay; Suchandra Mukherjee; Kenneth Iregbu; Fatima Modibbo; Stella Uwaezuoke; Rabaab Zahra; Haider Shirazi; Adil Muhammad; Jean-Baptiste Mazarati; Aniceth Rucogoza; Lucie Gaju; Shaheen Mehtar; Andre N H Bulabula; Andrew Whitelaw; Timothy R Walsh
Journal:  Nat Microbiol       Date:  2021-03-29       Impact factor: 17.745

Review 3.  Early-Onset Neonatal Sepsis in Low- and Middle-Income Countries: Current Challenges and Future Opportunities.

Authors:  Kirsty Sands; Owen B Spiller; Kathryn Thomson; Edward A R Portal; Kenneth C Iregbu; Timothy R Walsh
Journal:  Infect Drug Resist       Date:  2022-03-09       Impact factor: 4.003

4.  High Burden of Bloodstream Infections Associated With Antimicrobial Resistance and Mortality in the Neonatal Intensive Care Unit in Pune, India.

Authors:  Julia Johnson; Matthew L Robinson; Uday C Rajput; Chhaya Valvi; Aarti Kinikar; Tushar B Parikh; Umesh Vaidya; Sudhir Malwade; Sharad Agarkhedkar; Bharat Randive; Abhay Kadam; Rachel M Smith; Matthew Westercamp; Vidya Mave; Amita Gupta; Aaron M Milstone; Yukari C Manabe
Journal:  Clin Infect Dis       Date:  2021-07-15       Impact factor: 20.999

5.  Outbreak investigation of Serratia marcescens neurosurgical site infections associated with a contaminated shaving razors.

Authors:  Eun Jin Kim; Wan Beom Park; Jung-Ki Yoon; Won-Sang Cho; Su Jung Kim; Young Rok Oh; Kang Il Jun; Chang Kyung Kang; Pyeong Gyun Choe; Jong-Il Kim; Eun Hwa Choi; Myoung Don Oh; Nam Joong Kim
Journal:  Antimicrob Resist Infect Control       Date:  2020-05-12       Impact factor: 4.887
  5 in total

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