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Literature DB >> 30838581

The Expression and Purification of Recombinant Mouse Ameloblastin in E. coli.

Jingtan Su¹, Rucha Arun Bapat¹, Janet Moradian-Oldak^2,3.

Abstract

Ameloblastin is the second most abundant enamel matrix protein, and is thought to be essential for ameloblast cell polarization, cell adhesion, and enamel mineralization. However, studies of ameloblastin's function and its molecular mechanism have been limited due to difficulty in obtaining recombinant ameloblastin in vitro. Here, we present a protocol for successful ameloblastin expression and purification in E. coli.

Entities: CellLine Chemical Disease Gene Species

Keywords: Ameloblastin; Enamel; Expression and purification; Matrix protein

Mesh：

Substances：

Year: 2019 PMID： 30838581 PMCID： PMC8011534 DOI： 10.1007/978-1-4939-9012-2_23

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

15 in total

1. Synthesis, secretion, degradation, and fate of ameloblastin during the matrix formation stage of the rat incisor as shown by immunocytochemistry and immunochemistry using region-specific antibodies.

Authors: T Uchida; C Murakami; N Dohi; K Wakida; T Satoda; O Takahashi
Journal: J Histochem Cytochem Date: 1997-10 Impact factor: 2.479

2. Intrinsically disordered proteins drive enamel formation via an evolutionarily conserved self-assembly motif.

Authors: Tomas Wald; Frantisek Spoutil; Adriana Osickova; Michaela Prochazkova; Oldrich Benada; Petr Kasparek; Ladislav Bumba; Ophir D Klein; Radislav Sedlacek; Peter Sebo; Jan Prochazka; Radim Osicka
Journal: Proc Natl Acad Sci U S A Date: 2017-02-14 Impact factor: 11.205

3. Immunochemical and immunohistochemical study of the 27- and 29-kDa calcium-binding proteins and related proteins in the porcine tooth germ.

Authors: C Murakami; N Dohi; M Fukae; T Tanabe; Y Yamakoshi; K Wakida; T Satoda; O Takahashi; M Shimizu; O H Ryu; J P Simmer; T Uchida
Journal: Histochem Cell Biol Date: 1997-06 Impact factor: 4.304

4. The Importance of Serine Phosphorylation of Ameloblastin on Enamel Formation.

Authors: P Ma; W Yan; Y Tian; J He; S J Brookes; X Wang
Journal: J Dent Res Date: 2016-07-29 Impact factor: 6.116

5. Cleavage site specificity of MMP-20 for secretory-stage ameloblastin.

Authors: Y-H P Chun; Y Yamakoshi; F Yamakoshi; M Fukae; J C-C Hu; J D Bartlett; J P Simmer
Journal: J Dent Res Date: 2010-04-16 Impact factor: 6.116

6. Ameloblastin gene (AMBN) maps within the critical region for autosomal dominant amelogenesis imperfecta at chromosome 4q21.

Authors: M MacDougall; B R DuPont; D Simmons; B Reus; P Krebsbach; C Kärrman; G Holmgren; R J Leach; K Forsman
Journal: Genomics Date: 1997-04-01 Impact factor: 5.736

7. Interaction between the enamel matrix proteins amelogenin and ameloblastin.

Authors: Hanumanth H Ravindranath; Li-Sha Chen; Margaret Zeichner-David; Rieko Ishima; Rajeswari M H Ravindranath
Journal: Biochem Biophys Res Commun Date: 2004-10-22 Impact factor: 3.575

Review 8. Protein-mediated enamel mineralization.

Authors: Janet Moradian-Oldak
Journal: Front Biosci (Landmark Ed) Date: 2012-06-01

9. Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts.

Authors: Satoshi Fukumoto; Takayoshi Kiba; Bradford Hall; Noriyuki Iehara; Takashi Nakamura; Glenn Longenecker; Paul H Krebsbach; Antonio Nanci; Ashok B Kulkarni; Yoshihiko Yamada
Journal: J Cell Biol Date: 2004-12-06 Impact factor: 10.539

10. Evolutionary analysis of selective constraints identifies ameloblastin (AMBN) as a potential candidate for amelogenesis imperfecta.

Authors: Frédéric Delsuc; Barbara Gasse; Jean-Yves Sire
Journal: BMC Evol Biol Date: 2015-07-30 Impact factor: 3.260

4 in total