Literature DB >> 30838561

Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells.

Elizabeth Guirado1, Youbin Zhang1, Anne George2.   

Abstract

This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments.

Entities:  

Keywords:  Gene transfer techniques; Genetic recombination; Genetic transduction; Lentivirus; Somatic stem cells

Mesh：

Year:  2019        PMID： 30838561      PMCID： PMC6500575          DOI： 10.1007/978-1-4939-9012-2_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.

Authors:  S Gronthos; M Mankani; J Brahim; P G Robey; S Shi
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

2.  Odontoblast differentiation of human dental pulp cells in explant cultures.

Authors:  M L Couble; J C Farges; F Bleicher; B Perrat-Mabillon; M Boudeulle; H Magloire
Journal:  Calcif Tissue Int       Date:  2000-02       Impact factor: 4.333

3.  Small- to large-scale production of lentivirus vectors.

Authors:  Michael P Marino; Milson J Luce; Jakob Reiser
Journal:  Methods Mol Biol       Date:  2003

4.  Collagen gene expression in human dental pulp cell cultures.

Authors:  M Y Kuo; W H Lan; S K Lin; K S Tsai; L J Hahn
Journal:  Arch Oral Biol       Date:  1992-11       Impact factor: 2.633

5.  Advanced zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments.

Authors:  Christian Mosimann; Leonard I Zon
Journal:  Methods Cell Biol       Date:  2011       Impact factor: 1.441

6.  Calcification capacity of dental papilla mesenchymal cells transplanted in the isogenic mouse spleen.

Authors:  K Ishizeki; T Nawa; M Sugawara
Journal:  Anat Rec       Date:  1990-03

Review 7.  Retrovirus packaging cells.

Authors:  A D Miller
Journal:  Hum Gene Ther       Date:  1990       Impact factor: 5.695
  7 in total
  1 in total

Review 1.  Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration.

Authors:  Sarah Hani Shoushrah; Janis Lisa Transfeld; Christian Horst Tonk; Dominik Büchner; Steffen Witzleben; Martin A Sieber; Margit Schulze; Edda Tobiasch
Journal:  Int J Mol Sci       Date:  2021-06-15       Impact factor: 5.923
  1 in total

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