Wei Liu1, Junmeng Zheng2, Jianzeng Dong1, Rong Bai1, Deli Song1, Xiaohai Ma3, Lei Zhao3, Yan Yao1, Haibo Zhang4, Tong Liu5. 1. Department of Cardiology, Beijing AnZhen Hospital, Capital Medical University, Beijing, China. 2. Department of Cardiothoracic Surgery, Second Affiliated Hospital of Sun Yat-Sen University, Guang Dong, China. 3. Department of Radiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China. 4. Department of Cardiovascular Surgery, Beijing AnZhen Hospital, Capital Medical University, Beijing, China. 5. Department of Cardiology, Beijing AnZhen Hospital, Capital Medical University, Beijing, China, ltanzhen@126.com.
Abstract
OBJECTIVE: The aim of this study was to identify heart failure (HF)-specific circulating micro-RNAs (miRNA), and examine whether the selected miRNAs correlate with myocardial fibrosis and are reflective of the incidence of adverse cardiovascular events in patients with stage C or D HF. METHODS: Circulating miRNAs which were expressed in end-stage HF patients and matched healthy controls were detected by microarray analysis and validated by quantitative real-time polymerase chain reaction. Multivariate Cox regression analysis was performed to determine whether the selected circulating miRNAs could be prognostic factors in HF patients. RESULTS: In a cohort of 7 healthy controls and 9 patients with stage C or D HF, 7 miRNAs were differentially expressed. These miRNAs were further investigated in a second cohort of 80 patients with stage C or D HF and 30 healthy controls. Only miR-197-5P correlated with fibrosis as seen in cardiac magnetic resonance imaging in patients under the age of 50 years with stage C or D HF (r = 0.42, p = 0.008). Multivariate analyses revealed that miR-197-5P was also a risk factor for composite endpoint events in patients under the age of 50 years with stage C or D HF. CONCLUSION: miR-197-5P is a circulation miRNA that correlates with MF and adverse cardiac events in HF patients under the age of 50 years.
OBJECTIVE: The aim of this study was to identify heart failure (HF)-specific circulating micro-RNAs (miRNA), and examine whether the selected miRNAs correlate with myocardial fibrosis and are reflective of the incidence of adverse cardiovascular events in patients with stage C or D HF. METHODS: Circulating miRNAs which were expressed in end-stage HF patients and matched healthy controls were detected by microarray analysis and validated by quantitative real-time polymerase chain reaction. Multivariate Cox regression analysis was performed to determine whether the selected circulating miRNAs could be prognostic factors in HF patients. RESULTS: In a cohort of 7 healthy controls and 9 patients with stage C or D HF, 7 miRNAs were differentially expressed. These miRNAs were further investigated in a second cohort of 80 patients with stage C or D HF and 30 healthy controls. Only miR-197-5P correlated with fibrosis as seen in cardiac magnetic resonance imaging in patients under the age of 50 years with stage C or D HF (r = 0.42, p = 0.008). Multivariate analyses revealed that miR-197-5P was also a risk factor for composite endpoint events in patients under the age of 50 years with stage C or D HF. CONCLUSION:miR-197-5P is a circulation miRNA that correlates with MF and adverse cardiac events in HF patients under the age of 50 years.
Authors: Claudia Sacchetto; Zenab Mohseni; Robin M W Colpaert; Libero Vitiello; Marzia De Bortoli; Indira G C Vonhögen; Ke Xiao; Giulia Poloni; Alessandra Lorenzon; Chiara Romualdi; Riccardo Bariani; Elisa Mazzotti; Luciano Daliento; Barbara Bauce; Domenico Corrado; Thomas Thum; Alessandra Rampazzo; Leon J de Windt; Martina Calore Journal: Cells Date: 2021-09-28 Impact factor: 6.600