| Literature DB >> 30835535 |
Paramanantham Parasuraman1, Asha P Antony1, Sruthil Lal S B2, Alok Sharan2, Busi Siddhardha1, Kaviyarasu Kasinathan3,4, Needa A Bahkali5, Turki M S Dawoud6, Asad Syed6.
Abstract
In the present study, the antimicrobial and antibiofilm efficacy of toluidine blue (TB) encapsulated in mesoporous silica nanoparticles (MSN) was investigated against Pseudomonas aeruginosa and Staphylococcus aureus treated with antimicrobial photodynamic therapy (aPDT) using a red diode laser 670 nm wavelength, 97.65 J cm-2 radiant exposure, 5 min). Physico-chemical techniques (UV-visible (UV-vis) absorption, photoluminescence emission, excitation, and FTIR) and high-resolution transmission electron microscopy (HR-TEM) were employed to characterize the conjugate of TB encapsulated in MSN (TB MSN). TB MSN showed maximum antimicrobial activities corresponding to 5.03 and 5.56 log CFU ml-1 reductions against P. aeruginosa and S. aureus, respectively, whereas samples treated with TB alone showed 2.36 and 2.66 log CFU ml-1 reductions. Anti-biofilm studies confirmed that TB MSN effectively inhibits biofilm formation and production of extracellular polymeric substances by P. aeruginosa and S. aureus.Entities:
Keywords: Antimicrobial photodynamic therapy; anti-biofilm; encapsulation; mesoporous silica nanoparticles; photosensitizer; reactive oxygen species; toluidine blue
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Year: 2019 PMID： 30835535 DOI： 10.1080/08927014.2019.1570501
Source DB: PubMed Journal: Biofouling ISSN： 0892-7014 Impact factor: 3.209