Literature DB >> 30833354

Expanding the Clostridioides difficile Genetics Toolbox.

Aimee Shen1.   

Abstract

Clostridioides difficile genetics has rapidly advanced in recent years thanks to the development of tools for allelic replacement and transposon mutagenesis. In this Journal of Bacteriology issue, Müh et al extend the genetics toolbox by developing a CRISPRi strategy for gene silencing in C. difficile (U.Müh, A. G. Pannullo, D. S. Weiss, and C. D. Ellermeier, 2019, J Bacteriol 201:e00711-18. . https://doi.org/10.1128/JB.00711-18). The authors demonstrate the tunability and robustness of their CRISPRi system, highlight its utility in studying essential gene function, and discuss exciting new possibilities for dissecting C. difficile physiology.
Copyright © 2019 American Society for Microbiology.

Entities:  

Year:  2019        PMID： 30833354      PMCID： PMC6597399          DOI： 10.1128/JB.00089-19

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  33 in total

1.  Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier.

Authors:  Des Purdy; Triona A T O'Keeffe; Michael Elmore; Mike Herbert; Anne McLeod; Monika Bokori-Brown; Anna Ostrowski; Nigel P Minton
Journal:  Mol Microbiol       Date:  2002-10       Impact factor: 3.501

2.  The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome.

Authors:  Mohammed Sebaihia; Brendan W Wren; Peter Mullany; Neil F Fairweather; Nigel Minton; Richard Stabler; Nicholas R Thomson; Adam P Roberts; Ana M Cerdeño-Tárraga; Hongmei Wang; Matthew T G Holden; Anne Wright; Carol Churcher; Michael A Quail; Stephen Baker; Nathalie Bason; Karen Brooks; Tracey Chillingworth; Ann Cronin; Paul Davis; Linda Dowd; Audrey Fraser; Theresa Feltwell; Zahra Hance; Simon Holroyd; Kay Jagels; Sharon Moule; Karen Mungall; Claire Price; Ester Rabbinowitsch; Sarah Sharp; Mark Simmonds; Kim Stevens; Louise Unwin; Sally Whithead; Bruno Dupuy; Gordon Dougan; Bart Barrell; Julian Parkhill
Journal:  Nat Genet       Date:  2006-06-25       Impact factor: 38.330

3.  Clostridium difficile has two parallel and essential Sec secretion systems.

Authors:  Robert P Fagan; Neil F Fairweather
Journal:  J Biol Chem       Date:  2011-06-09       Impact factor: 5.157

4.  Cefoperazone-treated mice as an experimental platform to assess differential virulence of Clostridium difficile strains.

Authors:  Casey M Theriot; Charles C Koumpouras; Paul E Carlson; Ingrid I Bergin; David M Aronoff; Vincent B Young
Journal:  Gut Microbes       Date:  2011-11-01

5.  Environmental response and autoregulation of Clostridium difficile TxeR, a sigma factor for toxin gene expression.

Authors:  Nagraj Mani; Dena Lyras; Lisa Barroso; Pauline Howarth; Tracy Wilkins; Julian I Rood; Abraham L Sonenshein; Bruno Dupuy
Journal:  J Bacteriol       Date:  2002-11       Impact factor: 3.490

6.  Construction and analysis of chromosomal Clostridium difficile mutants.

Authors:  Jennifer R O'Connor; Dena Lyras; Kylie A Farrow; Vicki Adams; David R Powell; Jason Hinds; Jackie K Cheung; Julian I Rood
Journal:  Mol Microbiol       Date:  2006-09       Impact factor: 3.501

7.  A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.

Authors:  R Allyn Forsyth; Robert J Haselbeck; Kari L Ohlsen; Robert T Yamamoto; Howard Xu; John D Trawick; Daniel Wall; Liangsu Wang; Vickie Brown-Driver; Jamie M Froelich; Kedar G C; Paula King; Melissa McCarthy; Cheryl Malone; Brian Misiner; David Robbins; Zehui Tan; Zhan-yang Zhu Zy; Grant Carr; Deborah A Mosca; Carlos Zamudio; J Gordon Foulkes; Judith W Zyskind
Journal:  Mol Microbiol       Date:  2002-03       Impact factor: 3.501

8.  Generation of an erythromycin-sensitive derivative of Clostridium difficile strain 630 (630Deltaerm) and demonstration that the conjugative transposon Tn916DeltaE enters the genome of this strain at multiple sites.

Authors:  Haitham A Hussain; Adam P Roberts; Peter Mullany
Journal:  J Med Microbiol       Date:  2005-02       Impact factor: 2.472

9.  Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium.

Authors:  Richard A Stabler; Miao He; Lisa Dawson; Melissa Martin; Esmeralda Valiente; Craig Corton; Trevor D Lawley; Mohammed Sebaihia; Michael A Quail; Graham Rose; Dale N Gerding; Maryse Gibert; Michel R Popoff; Julian Parkhill; Gordon Dougan; Brendan W Wren
Journal:  Genome Biol       Date:  2009-09-25       Impact factor: 13.583

10.  The ClosTron: a universal gene knock-out system for the genus Clostridium.

Authors:  John T Heap; Oliver J Pennington; Stephen T Cartman; Glen P Carter; Nigel P Minton
Journal:  J Microbiol Methods       Date:  2007-06-18       Impact factor: 2.363
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  2 in total

Review 1.  25th Annual Midwest Microbial Pathogenesis Conference

Authors:  Timothy L Yahr; Craig D Ellermeier
Journal:  J Bacteriol       Date:  2019-06-21       Impact factor: 3.490

2.  A cortex-specific penicillin-binding protein contributes to heat resistance in Clostridioides difficile spores.

Authors:  Yasir Adil Jabbar Alabdali; Peter Oatley; Joseph A Kirk; Robert P Fagan
Journal:  Anaerobe       Date:  2021-04-30       Impact factor: 3.331
  2 in total

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