John M Wentworth1,2, Naiara G Bediaga3,4, Megan A S Penno5, Esther Bandala-Sanchez3,4, Komal N Kanojia6, Konstantinos A Kouremenos6, Jennifer J Couper5, Leonard C Harrison3,4. 1. Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. wentworth@wehi.edu.au. 2. Department of Medical Biology, University of Melbourne, Parkville, VIC, 3010, Australia. wentworth@wehi.edu.au. 3. Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. 4. Department of Medical Biology, University of Melbourne, Parkville, VIC, 3010, Australia. 5. Robinson Research Institute and Adelaide Medical School, University of Adelaide, Adelaide, SA, Australia. 6. Metabolomics Australia, Bio21 Institute, University of Melbourne, Parkville, VIC, Australia.
Abstract
BACKGROUND: Cord blood lipids are potential disease biomarkers. We aimed to determine if their concentrations were affected by delayed blood processing. METHOD: Refrigerated cord blood from six healthy newborns was centrifuged every 12 h for 4 days. Plasma lipids were analysed by liquid chromatography/mass spectroscopy. RESULTS: Of 262 lipids identified, only eight varied significantly over time. These comprised three dihexosylceramides, two phosphatidylserines and two phosphatidylethanolamines whose relative concentrations increased and one sphingomyelin that decreased. CONCLUSION: Delay in separation of plasma from refrigerated cord blood has minimal effect overall on the plasma lipidome.
BACKGROUND: Cord blood lipids are potential disease biomarkers. We aimed to determine if their concentrations were affected by delayed blood processing. METHOD: Refrigerated cord blood from six healthy newborns was centrifuged every 12 h for 4 days. Plasma lipids were analysed by liquid chromatography/mass spectroscopy. RESULTS: Of 262 lipids identified, only eight varied significantly over time. These comprised three dihexosylceramides, two phosphatidylserines and two phosphatidylethanolamines whose relative concentrations increased and one sphingomyelin that decreased. CONCLUSION: Delay in separation of plasma from refrigerated cord blood has minimal effect overall on the plasma lipidome.
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