Adlin Afzan1,2, Noraini Kasim3, Nor Hadiani Ismail3, Norfaizura Azmi4, Abdul Manaf Ali4,5,6, Nashriyah Mat4, Jean-Luc Wolfender7. 1. School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Rue Michel-Servet 1, 1206, Geneva, Switzerland. 2. Phytochemistry Unit, Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang, 50588, Kuala Lumpur, Malaysia. 3. Atta-ur-Rahman Institute for Natural Products Discovery, Universiti Teknologi MARA, Kampus Puncak Alam, 42300, Selangor, Malaysia. 4. Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200, Besut, Terengganu, Malaysia. 5. Institute of Agropolis, Universiti Sultan Zainal Abidin, Campus Besut, 22200, Besut, Terengganu, Malaysia. 6. Natural Medicine Center, Universiti Islam, Cyberjaya, 63000, Cyberjaya, Selangor, Malaysia. 7. School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Rue Michel-Servet 1, 1206, Geneva, Switzerland. Jean-Luc.Wolfender@unige.ch.
Abstract
BACKGROUND: Ficus deltoidea Jack (Moraceae) is a plant used in Malaysia for various diseases including as a supplement in diabetes management. Morphology distinction of the 7 main varieties (var. angustifolia, var. bilobata, var. deltoidea, var. intermedia, var. kunstleri, var. motleyana and var. trengganuensis) is challenging due to the extreme leaf heterophylly and unclear varietal boundaries, making it difficult for quality control of F. deltoidea products. OBJECTIVE: We aimed to compare the phytochemical composition of 7 varieties growing in different conditions at various geographical locations. We also aimed to establish the quality control markers for the authentication of these varieties. METHODS: We applied untargeted UHPLC-TOFMS metabolomics to discriminate 100 leaf samples of F. deltoidea collected from 6 locations in Malaysia. A genetic analysis on 21 leaf samples was also performed to validate the chemotaxonomy differentiation. RESULTS: The PCA and HCA analysis revealed the existence of 3 chemotypes based on the differentiation in the flavonoid content. The PLS-DA analysis identified 15 glycosylated flavone markers together with 1 furanocoumarin. These markers were always consistent for the respective varieties, regardless of the geographical locations and growing conditions. The chemotaxonomy differentiation was in agreement with the DNA sequencing. In particular, var. bilobata accession which showed divergent morphology was also differentiated by the chemical fingerprints and genotype. CONCLUSION: Chemotype differentiation based on the flavonoid fingerprints along with the proposed markers provide a powerful identification tool to complement morphology and genetic analyses for the quality control of raw materials and products from F. deltoidea.
BACKGROUND:Ficus deltoidea Jack (Moraceae) is a plant used in Malaysia for various diseases including as a supplement in diabetes management. Morphology distinction of the 7 main varieties (var. angustifolia, var. bilobata, var. deltoidea, var. intermedia, var. kunstleri, var. motleyana and var. trengganuensis) is challenging due to the extreme leaf heterophylly and unclear varietal boundaries, making it difficult for quality control of F. deltoidea products. OBJECTIVE: We aimed to compare the phytochemical composition of 7 varieties growing in different conditions at various geographical locations. We also aimed to establish the quality control markers for the authentication of these varieties. METHODS: We applied untargeted UHPLC-TOFMS metabolomics to discriminate 100 leaf samples of F. deltoidea collected from 6 locations in Malaysia. A genetic analysis on 21 leaf samples was also performed to validate the chemotaxonomy differentiation. RESULTS: The PCA and HCA analysis revealed the existence of 3 chemotypes based on the differentiation in the flavonoid content. The PLS-DA analysis identified 15 glycosylated flavone markers together with 1 furanocoumarin. These markers were always consistent for the respective varieties, regardless of the geographical locations and growing conditions. The chemotaxonomy differentiation was in agreement with the DNA sequencing. In particular, var. bilobata accession which showed divergent morphology was also differentiated by the chemical fingerprints and genotype. CONCLUSION: Chemotype differentiation based on the flavonoid fingerprints along with the proposed markers provide a powerful identification tool to complement morphology and genetic analyses for the quality control of raw materials and products from F. deltoidea.
Entities:
Keywords:
Chemical marker; Chemotaxonomy; DNA; Ficus deltoidea; MS metabolomics; Phylogenetic; Quality control
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