Jisun H J Lee1, G K Jayaprakasha1, Charlie M Rush2, Kevin M Crosby3, Bhimanagouda S Patil4. 1. Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. 2. Plant Pathology, Texas A&M AgriLife Research, and Extension, Amarillo Research & Extension Center, 6500 Amarillo Boulevard West, Amarillo, TX, 79106, USA. 3. Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. k-crosby@tamu.edu. 4. Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, A120, College Station, TX, 77845-2119, USA. b-patil@tamu.edu.
Abstract
INTRODUCTION: In recent years, growers have used various production types, including high-tunnel systems, to increase the yield of tomatoes (Lycopersicon esculentum). However, the effect of high-tunnel cultivation, in comparison to conventional open-field production, on aroma and flavor volatiles is not fully understood. OBJECTIVES: To optimize the extraction and quantification conditions for the analysis of tomato volatiles using headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS), and study the effect of production systems on volatile profiles using metabolomics approach. METHODS: The HS-SPME conditions were optimized for extraction and GC-MS was used to quantify the volatiles from four tomato varieties grown in open-field and high-tunnel systems. Univariate and multivariate analyses were performed to identify the influence of production system on tomato volatiles. RESULTS AND CONCLUSIONS: The extraction of 2 g tomato samples at 60 °C for 45 min using divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber gave the maximum amounts of volatiles. This optimized method was used to identify and quantify 41 volatiles from four tomato varieties. The levels of β-damascenone were higher in the high-tunnel tomatoes and geranylacetone was higher in open-field tomatoes. These two volatile compounds could be considered as biomarkers for tomatoes grown in high-tunnel and open-field production systems. This study is the first report comparing volatiles in tomatoes grown in high-tunnel and open-field conditions, and our results confirmed that there is a critical need to adopt biomarker-specific production systems to improve the nutritional and organoleptic properties of tomatoes.
INTRODUCTION: In recent years, growers have used various production types, including high-tunnel systems, to increase the yield of tomatoes (Lycopersicon esculentum). However, the effect of high-tunnel cultivation, in comparison to conventional open-field production, on aroma and flavor volatiles is not fully understood. OBJECTIVES: To optimize the extraction and quantification conditions for the analysis of tomato volatiles using headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS), and study the effect of production systems on volatile profiles using metabolomics approach. METHODS: The HS-SPME conditions were optimized for extraction and GC-MS was used to quantify the volatiles from four tomato varieties grown in open-field and high-tunnel systems. Univariate and multivariate analyses were performed to identify the influence of production system on tomato volatiles. RESULTS AND CONCLUSIONS: The extraction of 2 g tomato samples at 60 °C for 45 min using divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber gave the maximum amounts of volatiles. This optimized method was used to identify and quantify 41 volatiles from four tomato varieties. The levels of β-damascenone were higher in the high-tunnel tomatoes and geranylacetone was higher in open-field tomatoes. These two volatile compounds could be considered as biomarkers for tomatoes grown in high-tunnel and open-field production systems. This study is the first report comparing volatiles in tomatoes grown in high-tunnel and open-field conditions, and our results confirmed that there is a critical need to adopt biomarker-specific production systems to improve the nutritional and organoleptic properties of tomatoes.
Authors: Denise Tieman; Guangtao Zhu; Marcio F R Resende; Tao Lin; Cuong Nguyen; Dawn Bies; Jose Luis Rambla; Kristty Stephanie Ortiz Beltran; Mark Taylor; Bo Zhang; Hiroki Ikeda; Zhongyuan Liu; Josef Fisher; Itay Zemach; Antonio Monforte; Dani Zamir; Antonio Granell; Matias Kirst; Sanwen Huang; Harry Klee Journal: Science Date: 2017-01-27 Impact factor: 47.728
Authors: Brian Farneti; Alberto Algarra Alarcón; Fotios G Papasotiriou; D Samudrala; Simona M Cristescu; Guglielmo Costa; Frans J M Harren; Ernst J Woltering Journal: Food Bioproc Tech Date: 2015-03-25 Impact factor: 4.465
Authors: Amelia C Peterson; Jan-Peter Hauschild; Scott T Quarmby; Dirk Krumwiede; Oliver Lange; Rachelle A S Lemke; Florian Grosse-Coosmann; Stevan Horning; Timothy J Donohue; Michael S Westphall; Joshua J Coon; Jens Griep-Raming Journal: Anal Chem Date: 2014-09-10 Impact factor: 6.986
Authors: Deepak M Kasote; G K Jayaprakasha; Kevin Ong; Kevin M Crosby; Bhimanagouda S Patil Journal: BMC Plant Biol Date: 2020-10-22 Impact factor: 4.215
Authors: Eric de Castro Tobaruela; Bruna Lima Gomes; Vanessa Caroline de Barros Bonato; Elis Silva de Lima; Luciano Freschi; Eduardo Purgatto Journal: Front Plant Sci Date: 2021-12-10 Impact factor: 5.753