Farhana R Pinu1, Ninna Granucci2, James Daniell2,3, Ting-Li Han2, Sonia Carneiro4, Isabel Rocha4, Jens Nielsen5,6, Silas G Villas-Boas2. 1. The New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland, 1142, New Zealand. Farhana.Pinu@plantandfood.co.nz. 2. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. 3. LanzaTech, Skokie, IL, 60077, USA. 4. Center of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal. 5. Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96, Gothenburg, Sweden. 6. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2970, Hørsholm, Denmark.
Abstract
INTRODUCTION: Microbial cells secrete many metabolites during growth, including important intermediates of the central carbon metabolism. This has not been taken into account by researchers when modeling microbial metabolism for metabolic engineering and systems biology studies. MATERIALS AND METHODS: The uptake of metabolites by microorganisms is well studied, but our knowledge of how and why they secrete different intracellular compounds is poor. The secretion of metabolites by microbial cells has traditionally been regarded as a consequence of intracellular metabolic overflow. CONCLUSIONS: Here, we provide evidence based on time-series metabolomics data that microbial cells eliminate some metabolites in response to environmental cues, independent of metabolic overflow. Moreover, we review the different mechanisms of metabolite secretion and explore how this knowledge can benefit metabolic modeling and engineering.
INTRODUCTION: Microbial cells secrete many metabolites during growth, including important intermediates of the central carbon metabolism. This has not been taken into account by researchers when modeling microbial metabolism for metabolic engineering and systems biology studies. MATERIALS AND METHODS: The uptake of metabolites by microorganisms is well studied, but our knowledge of how and why they secrete different intracellular compounds is poor. The secretion of metabolites by microbial cells has traditionally been regarded as a consequence of intracellular metabolic overflow. CONCLUSIONS: Here, we provide evidence based on time-series metabolomics data that microbial cells eliminate some metabolites in response to environmental cues, independent of metabolic overflow. Moreover, we review the different mechanisms of metabolite secretion and explore how this knowledge can benefit metabolic modeling and engineering.
Entities:
Keywords:
Active efflux; Metabolic engineering; Metabolic modeling; Microbial metabolism; Microorganisms; Secretion; Systems biology
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