Ya-Li Zhang1, Chun-Ling Zhang1, Gui-Luan Wang1, Yong-Xu Wang1, Chen-Hui Qi1, Chun-Xiang You1, Yuan-Yuan Li2, Yu-Jin Hao3. 1. National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-an, 271018, Shandong, China. 2. National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-an, 271018, Shandong, China. liyy0912@163.com. 3. National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-an, 271018, Shandong, China. haoyujin@sdau.edu.cn.
Abstract
MAIN CONCLUSION: This study showed that AP2/EREBP transcription factor MdSHINE2 functioned in mediating cuticular permeability, sensitivity to abscisic acid (ABA), and drought resistance by regulating wax biosynthesis. Plant cuticular wax plays crucial roles in protecting plants from environmental stresses, particularly drought stress. Many enzymes and transcription factors involved in wax biosynthesis have been identified in plant species. In this study, we identified an AP2/EREBP transcription factor, MdSHINE2 from apple, which is a homolog of AtSHINE2 in Arabidopsis. MdSHINE2 was constitutively expressed at different levels in various apple tissues, and the transcription level of MdSHINE2 was induced substantially by abiotic stress and hormone treatments. MdSHINE2-overexpressing Arabidopsis exhibited great change in cuticular wax crystal numbers and morphology and wax composition of leaves and stems. Moreover, MdSHINE2 heavily influenced cuticular permeability, sensitivity to abscisic acid, and drought resistance.
MAIN CONCLUSION: This study showed that AP2/EREBP transcription factor MdSHINE2 functioned in mediating cuticular permeability, sensitivity to abscisic acid (ABA), and drought resistance by regulating wax biosynthesis. Plant cuticular wax plays crucial roles in protecting plants from environmental stresses, particularly drought stress. Many enzymes and transcription factors involved in wax biosynthesis have been identified in plant species. In this study, we identified an AP2/EREBP transcription factor, MdSHINE2 from apple, which is a homolog of AtSHINE2 in Arabidopsis. MdSHINE2 was constitutively expressed at different levels in various apple tissues, and the transcription level of MdSHINE2 was induced substantially by abiotic stress and hormone treatments. MdSHINE2-overexpressing Arabidopsis exhibited great change in cuticular wax crystal numbers and morphology and wax composition of leaves and stems. Moreover, MdSHINE2 heavily influenced cuticular permeability, sensitivity to abscisic acid, and drought resistance.
Authors: Hafiz Muhammad Rizwan; Abdul Waheed; Songfeng Ma; Jiankun Li; Muhammad Bilal Arshad; Muhammad Irshad; Binqi Li; Xuelian Yang; Ahmad Ali; Mohamed A A Ahmed; Nusrat Shaheen; Sandra S Scholz; Ralf Oelmüller; Zhimin Lin; Faxing Chen Journal: Front Plant Sci Date: 2022-06-27 Impact factor: 6.627
Authors: Damiano Martignago; Andrés Rico-Medina; David Blasco-Escámez; Juan B Fontanet-Manzaneque; Ana I Caño-Delgado Journal: Front Plant Sci Date: 2020-01-22 Impact factor: 5.753