Literature DB >> 30824937

Adipose tissue-derived stem cells boost vascularization in grafted ovarian tissue by growth factor secretion and differentiation into endothelial cell lineages.

D D Manavella1, L Cacciottola1, V L Payen2, C A Amorim1, J Donnez3, M M Dolmans1,4.   

Abstract

Adipose tissue-derived stem cells (ASCs) have multilineage differentiation potential, proangiogenic properties, and the ability to enhance vascularization in xenografted human ovarian tissue. The aim of the present study was to identify the mechanisms behind the proangiogenic effects of ASCs. For this purpose, severe combined immunodeficient (SCID) mice were grafted with frozen-thawed human ovarian tissue. ASCs were labeled by lentiviral transfection for expression of enhanced green fluorescent protein (eGFP), and human ovarian tissue was grafted using a previously described two-step procedure. In the control group, ovarian tissue was transplanted using the standard one-step approach. Samples were collected and analyzed after 7 days. Detection of the eGFP antigen by immunofluorescence showed ASCs surrounding and infiltrating ovarian tissue grafts. Significantly higher vessel density was observed in the ASC group (P = 0.0182 versus control) on Day 7. Co-expression of eGFP, CD34 and CD31 was demonstrated in human vessels, confirming ASC differentiation into human endothelial cell lineages. Increased gene expression of vascular endothelial growth factor (VEGF) was also shown in the ASC group (P = 0.0182 versus control). Immunohistochemistry targeting anti-human VEGF revealed significantly higher expression levels in the ASC group (P = 0.033 versus control), while VEGF and eGFP immunofluorescence showed greater growth factor expression in areas surrounding ASCs. In conclusion, ASCs differentiate into human vessels and promote secretion of VEGF when transplanted together with human ovarian tissue to SCID mouse peritoneum using a two-step ovarian tissue grafting procedure. This is a promising step towards potentially improving ovarian tissue quality and lifespan. Long-term studies should be conducted to investigate ASC safety and efficacy in the context of ovarian tissue transplantation.
© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Entities:  

Keywords:  adipose tissue-derived stem cells; differentiation; endothelial cells; fibroblast growth factor; ovarian tissue transplantation; vascular endothelial growth factor

Mesh：

Substances：

Year:  2019        PMID： 30824937     DOI： 10.1093/molehr/gaz008

Source DB:  PubMed          Journal:  Mol Hum Reprod        ISSN： 1360-9947            Impact factor:   4.025


  11 in total

1.  Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications.

Authors:  Janice de M V Vilela; Saeid Moghassemi; Arezoo Dadashzadeh; Marie-Madeleine Dolmans; Ricardo B Azevedo; Christiani A Amorim
Journal:  Nanomaterials (Basel)       Date:  2022-06-10       Impact factor: 5.719

2.  Can frozen-thawed human ovary withstand refreezing-rethawing in the form of cortical strips?

Authors:  Camille Hossay; Alessandra Camboni; Luciana Cacciottola; Thu Y T Nguyen; Rossella Masciangelo; Jacques Donnez; Marie-Madeleine Dolmans
Journal:  J Assist Reprod Genet       Date:  2020-10-06       Impact factor: 3.412

3.  Adipose tissue-derived stem cells protect the primordial follicle pool from both direct follicle death and abnormal activation after ovarian tissue transplantation.

Authors:  Luciana Cacciottola; Guillaume E Courtoy; Thu Y T Nguyen; Camille Hossay; Jacques Donnez; Marie-Madeleine Dolmans
Journal:  J Assist Reprod Genet       Date:  2020-11-12       Impact factor: 3.412

Review 4.  Is It Possible to Treat Infertility with Stem Cells?

Authors:  P Petric; E Vrtacnik-Bokal; M Stimpfel
Journal:  Reprod Sci       Date:  2021-04-08       Impact factor: 3.060

Review 5.  The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis.

Authors:  Greg Hutchings; Krzysztof Janowicz; Lisa Moncrieff; Claudia Dompe; Ewa Strauss; Ievgeniia Kocherova; Mariusz J Nawrocki; Łukasz Kruszyna; Grzegorz Wąsiatycz; Paweł Antosik; Jamil A Shibli; Paul Mozdziak; Bartłomiej Perek; Zbigniew Krasiński; Bartosz Kempisty; Michał Nowicki
Journal:  Int J Mol Sci       Date:  2020-05-27       Impact factor: 5.923

6.  Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue.

Authors:  Shweta S Dipali; Christina R Ferreira; Luhan T Zhou; Michele T Pritchard; Francesca E Duncan
Journal:  Reprod Biol Endocrinol       Date:  2019-06-12       Impact factor: 5.211

7.  Long-Term Advantages of Ovarian Reserve Maintenance and Follicle Development Using Adipose Tissue-Derived Stem Cells in Ovarian Tissue Transplantation.

Authors:  Luciana Cacciottola; Thu Y T Nguyen; Maria C Chiti; Alessandra Camboni; Christiani A Amorim; Jacques Donnez; Marie-Madeleine Dolmans
Journal:  J Clin Med       Date:  2020-09-15       Impact factor: 4.241

8.  Autograft microskin combined with adipose-derived stem cell enhances wound healing in a full-thickness skin defect mouse model.

Authors:  Yuansen Luo; Xiaoyou Yi; Tangzhao Liang; Shihai Jiang; Ronghan He; Ying Hu; Li Bai; Chunmei Wang; Kun Wang; Lei Zhu
Journal:  Stem Cell Res Ther       Date:  2019-08-30       Impact factor: 6.832

Review 9.  Current and Future Perspectives for Improving Ovarian Tissue Cryopreservation and Transplantation Outcomes for Cancer Patients.

Authors:  Sanghoon Lee; Sinan Ozkavukcu; Seung-Yup Ku
Journal:  Reprod Sci       Date:  2021-03-31       Impact factor: 3.060

10.  Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture.

Authors:  Carmen Terren; Michelle Nisolle; Carine Munaut
Journal:  J Ovarian Res       Date:  2021-07-19       Impact factor: 4.234
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