Literature DB >> 30822456

Electron cryo-tomography of vestibular hair-cell stereocilia.

Zoltan Metlagel1, Jocelyn F Krey2, Junha Song1, Mark F Swift3, William J Tivol1, Rachel A Dumont2, Jasmine Thai1, Alex Chang1, Helia Seifikar1, Niels Volkmann3, Dorit Hanein3, Peter G Barr-Gillespie2, Manfred Auer4.   

Abstract

High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.
Copyright © 2019 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Actin; Cryo-electron microscopy; Hair cells; Stereocilia

Mesh：

Year:  2019        PMID： 30822456      PMCID： PMC6684322          DOI： 10.1016/j.jsb.2019.02.006

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  34 in total

1.  Actin cores of hair-cell stereocilia support myosin motility.

Authors:  G M Shepherd; D P Corey; S M Block
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  FEI's direct electron detector developments: Embarking on a revolution in cryo-TEM.

Authors:  Maarten Kuijper; Gerald van Hoften; Bart Janssen; Rudolf Geurink; Sacha De Carlo; Matthijn Vos; Gijs van Duinen; Bart van Haeringen; Marc Storms
Journal:  J Struct Biol       Date:  2015-10-09       Impact factor: 2.867

3.  Ultrastructural correlates of mechanoelectrical transduction in hair cells of the bullfrog's internal ear.

Authors:  R A Jacobs; A J Hudspeth
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1990

Review 4.  Hair cells: transduction, tuning, and transmission in the inner ear.

Authors:  W M Roberts; J Howard; A J Hudspeth
Journal:  Annu Rev Cell Biol       Date:  1988

5.  A portable cryo-plunger for on-site intact cryogenic microscopy sample preparation in natural environments.

Authors:  Luis R Comolli; Robert Duarte; Dennis Baum; Birgit Luef; Kenneth H Downing; David M Larson; Roseann Csencsits; Jillian F Banfield
Journal:  Microsc Res Tech       Date:  2011-12-30       Impact factor: 2.769

6.  Chemical dissection of stereovilli from fish inner ear reveals differences from intestinal microvilli.

Authors:  D C Neugebauer; U Thurm
Journal:  J Neurocytol       Date:  1984-10

7.  "Bundle blot" purification and initial protein characterization of hair cell stereocilia.

Authors:  G M Shepherd; B A Barres; D P Corey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

8.  Interactions between actin filaments and between actin filaments and membranes in quick-frozen and deeply etched hair cells of the chick ear.

Authors:  N Hirokawa; L G Tilney
Journal:  J Cell Biol       Date:  1982-10       Impact factor: 10.539

9.  Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.

Authors:  Jocelyn F Krey; Evan S Krystofiak; Rachel A Dumont; Sarath Vijayakumar; Dongseok Choi; Francisco Rivero; Bechara Kachar; Sherri M Jones; Peter G Barr-Gillespie
Journal:  J Cell Biol       Date:  2016-11-03       Impact factor: 10.539

10.  Flagellum couples cell shape to motility in Trypanosoma brucei.

Authors:  Stella Y Sun; Jason T Kaelber; Muyuan Chen; Xiaoduo Dong; Yasaman Nematbakhsh; Jian Shi; Matthew Dougherty; Chwee Teck Lim; Michael F Schmid; Wah Chiu; Cynthia Y He
Journal:  Proc Natl Acad Sci U S A       Date:  2018-06-11       Impact factor: 11.205
View more
  8 in total

1.  Cilia in the developing zebrafish ear.

Authors:  Tanya T Whitfield
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2019-12-30       Impact factor: 6.237

2.  Correction of Missing-Wedge Artifacts in Filamentous Tomograms by Template-Based Constrained Deconvolution.

Authors:  Julio Kovacs; Junha Song; Manfred Auer; Jing He; Wade Hunter; Willy Wriggers
Journal:  J Chem Inf Model       Date:  2020-03-19       Impact factor: 4.956

3.  Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia.

Authors:  Rui Gong; Fangfang Jiang; Zane G Moreland; Matthew J Reynolds; Santiago Espinosa de Los Reyes; Pinar Gurel; Arik Shams; James B Heidings; Michael R Bowl; Jonathan E Bird; Gregory M Alushin
Journal:  Sci Adv       Date:  2022-07-20       Impact factor: 14.957

4.  A cryo-tomography-based volumetric model of the actin core of mouse vestibular hair cell stereocilia lacking plastin 1.

Authors:  Junha Song; Roma Patterson; Zoltan Metlagel; Jocelyn F Krey; Samantha Hao; Linshanshan Wang; Brian Ng; Salim Sazzed; Julio Kovacs; Willy Wriggers; Jing He; Peter G Barr-Gillespie; Manfred Auer
Journal:  J Struct Biol       Date:  2020-01-18       Impact factor: 2.867

5.  Unveiling the polarity of actin filaments by cryo-electron tomography.

Authors:  Bruno Martins; Simona Sorrentino; Wen-Lu Chung; Meltem Tatli; Ohad Medalia; Matthias Eibauer
Journal:  Structure       Date:  2021-01-20       Impact factor: 5.006

6.  Molecular structures and conformations of protocadherin-15 and its complexes on stereocilia elucidated by cryo-electron tomography.

Authors:  Johannes Elferich; Sarah Clark; Jingpeng Ge; April Goehring; Aya Matsui; Eric Gouaux
Journal:  Elife       Date:  2021-12-29       Impact factor: 8.140

7.  Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy.

Authors:  Takushi Miyoshi; Thomas B Friedman; Naoki Watanabe
Journal:  STAR Protoc       Date:  2021-11-18

Review 8.  Human deafness-associated variants alter the dynamics of key molecules in hair cell stereocilia F-actin cores.

Authors:  Takushi Miyoshi; Inna A Belyantseva; Shin-Ichiro Kitajiri; Hiroki Miyajima; Shin-Ya Nishio; Shin-Ichi Usami; Bong Jik Kim; Byung Yoon Choi; Koichi Omori; Hari Shroff; Thomas B Friedman
Journal:  Hum Genet       Date:  2021-07-07       Impact factor: 4.132
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.