Adriana Morales-Martínez1,2, Absalom Zamorano-Carrillo2, Sergio Montes1, Mohammed El-Hafidi3, Alicia Sánchez-Mendoza4, Elizabeth Soria-Castro5, Juan Carlos Martínez-Lazcano6, Pablo Eliasib Martínez-Gopar1, Camilo Ríos1, Francisca Pérez-Severiano1. 1. Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Ciudad de México, México. 2. Laboratorio de Investigación de Bioquímica y Biofísica Computacional, ENMH, Instituto Politécnico Nacional, Ciudad de México, México. 3. Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México. 4. Departamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México. 5. Departamento de Patología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México. 6. Departamento de Neurofisiología, Instituto Nacional de Neurología y Neurocirugía, Ciudad de México, México.
Abstract
Background: Essential fatty acids (EFAs) and non-essential fatty acids (nEFAs) exert experimental and clinical neuroprotection in neurodegenerative diseases. The main EFAs, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), nEFAs, and oleic acid (OA) contained in olive and fish oils are inserted into the cell membranes, but the exact mechanism through which they exert neuroprotection is still unknown. Objectives and Methods: In this study, we assessed the fatty acids content and membrane fluidity in striatal rat synaptosomes after fatty acid-rich diets (olive- or a fish-oil diet, 15% w/w). Then, we evaluated the effect of enriching striatum synaptosomes with fatty acids on the oxidative damage produced by the prooxidants ferrous sulfate (FeSO4) or quinolinic acid (QUIN). Results and Discussion: Lipid profile analysis in striatal synaptosomes showed that EPA content increased in the fish oil group in comparison with control and olive groups. Furthermore, we found that synaptosomes enriched with fatty acids and incubated with QUIN or FeSO4 showed a significant oxidative damage reduction. Results suggest that EFAs, particularly EPA, improve membrane fluidity and confer antioxidant effect.
Background: Essential fatty acids (EFAs) and non-essential fatty acids (nEFAs) exert experimental and clinical neuroprotection in neurodegenerative diseases. The main EFAs, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), nEFAs, and oleic acid (OA) contained in olive and fish oils are inserted into the cell membranes, but the exact mechanism through which they exert neuroprotection is still unknown. Objectives and Methods: In this study, we assessed the fatty acids content and membrane fluidity in striatal rat synaptosomes after fatty acid-rich diets (olive- or a fish-oil diet, 15% w/w). Then, we evaluated the effect of enriching striatum synaptosomes with fatty acids on the oxidative damage produced by the prooxidants ferrous sulfate (FeSO4) or quinolinic acid (QUIN). Results and Discussion: Lipid profile analysis in striatal synaptosomes showed that EPA content increased in the fish oil group in comparison with control and olive groups. Furthermore, we found that synaptosomes enriched with fatty acids and incubated with QUIN or FeSO4 showed a significant oxidative damage reduction. Results suggest that EFAs, particularly EPA, improve membrane fluidity and confer antioxidant effect.
Authors: Adriana Morales-Martínez; Paola A Martínez-Gómez; Daniel Martinez-Fong; Marcos M Villegas-Rojas; Francisca Pérez-Severiano; Miguel A Del Toro-Colín; Karen M Delgado-Minjares; Víctor Manuel Blanco-Alvarez; Bertha Alicia Leon-Chavez; Omar Emiliano Aparicio-Trejo; Mauricio T Baéz-Cortés; Maria-Del-Carmen Cardenas-Aguayo; José Luna-Muñoz; Mar Pacheco-Herrero; Quetzalli D Angeles-López; Irma A Martínez-Dávila; Citlaltepetl Salinas-Lara; José Pablo Romero-López; Carlos Sánchez-Garibay; Adolfo R Méndez-Cruz; Luis O Soto-Rojas Journal: Int J Mol Sci Date: 2022-09-27 Impact factor: 6.208