| Literature DB >> 30820894 |
Marie-Theres Weil1,2,3, Giselheid Schulz-Ëberlin1, Chaitali Mukherjee4,5,6, Wen Ping Kuo-Elsner7, Isabelle Schäfer8, Christina Müller8, Mikael Simons9,10,11,12.
Abstract
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.Entities:
Keywords: Coculture; Magnetic-activated cell sorting; Oligodendrocyte differentiation; Oligodendrocyte precursor cells; Oligodendrocyte primary culture
Mesh：
Year: 2019 PMID： 30820894 DOI： 10.1007/978-1-4939-9072-6_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745