| Literature DB >> 30816099 |
O V Gnedenko1, E O Yablokov1, P V Ershov1, A V Svirid2, T V Shkel2, I V Haidukevich2, N V Strushkevich2, A A Gilep2, S A Usanov2, A S Ivanov1.
Abstract
Biosensor experiments on investigation of interaction between prostacyclin synthase (PGIS) and different proteins of the cytochrome P450 monooxygenase systems were perfomed. Interaction of PGIS with microsomal (CYP21A2, CYP2E1) and mitochondrial (CYP27A1, CYP11B1, CYP11B2, CYP11A1) cytochrome P450s was detected. Kinetic and equilibrium parameters of protein complexes formation were determined. Data obtained suggest an essential role of these hemoproteins interaction in regulation of prostacyclin and thromboxane A2 biosynthesis.Entities:
Keywords: cytochrome P450; optical biosensor Biacore; prostacyclin synthase (PGIS CYP8A1); surface plasmon resonance (SPR)
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Year: 2019 PMID： 30816099 DOI： 10.18097/PBMC20196501063
Source DB: PubMed Journal: Biomed Khim ISSN： 2310-6905