Isabelle Rohn1, Stefanie Raschke1, Michael Aschner2, Simon Tuck3, Doris Kuehnelt4, Anna Kipp5,6, Tanja Schwerdtle1,6, Julia Bornhorst1,6,7. 1. Institute of Nutritional Science, University of Potsdam, 14558, Nuthetal, Germany. 2. Albert Einstein College of Medicine, 10461, NY, USA. 3. Umeå Centre for Molecular Medicine, Umeå University, 90187, Umeå, Sweden. 4. Institute of Chemistry, Analytical Chemistry, NAWI Graz, University of Graz, 8010, Graz, Austria. 5. Institute of Nutrition, Friedrich Schiller University Jena, 07743, Jena, Germany. 6. TraceAge - DFG Research Unit FOR 2558, Berlin-Potsdam-Jena, Germany. 7. Faculty of Mathematics and Natural Sciences, University of Wuppertal, 42119, Wuppertal, Germany.
Abstract
SCOPE: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro- and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. METHODS AND RESULTS: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. CONCLUSION: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.
SCOPE: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro- and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. METHODS AND RESULTS: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. CONCLUSION:Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.
Authors: Julia Bornhorst; Sudipta Chakraborty; Sören Meyer; Hanna Lohren; Sigrid Grosse Brinkhaus; Adam L Knight; Kim A Caldwell; Guy A Caldwell; Uwe Karst; Tanja Schwerdtle; Aaron Bowman; Michael Aschner Journal: Metallomics Date: 2014-01-22 Impact factor: 4.526
Authors: Omamuyovwi M Ijomone; Priscila Gubert; Comfort O A Okoh; Alexandre M Varão; Leandro de O Amara; Oritoke M Aluko; Michael Aschner Journal: Neuromethods Date: 2021-07-24
Authors: Tiago Fonseca Silva; José Robson Neves Cavalcanti Filho; Mariana Mirelle Lima Barreto Fonsêca; Natalia Medeiros Dos Santos; Ana Carolina Barbosa da Silva; Adrielle Zagmignan; Afonso Gomes Abreu; Ana Paula Sant'Anna da Silva; Vera Lúcia de Menezes Lima; Nicácio Henrique da Silva; Lívia Macedo Dutra; Jackson Roberto Guedes da Silva Almeida; Márcia Vanusa da Silva; Maria Tereza Dos Santos Correia; Luís Cláudio Nascimento da Silva Journal: Pharmaceuticals (Basel) Date: 2020-03-16