Literature DB >> 30811086

The influence of serum-supplemented culture media in a transwell migration assay.

Siti Sarah Omar Zaki1, Livashini Kanesan1, Ming Yeh Danielle Leong1,2, Sharmili Vidyadaran1.   

Abstract

Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.
© 2019 International Federation for Cell Biology.

Entities:  

Keywords:  BV2 microglia; chemoattractant; serum; transwell migration assay

Year:  2019        PMID： 30811086     DOI： 10.1002/cbin.11122

Source DB:  PubMed          Journal:  Cell Biol Int        ISSN： 1065-6995            Impact factor:   3.612


  12 in total

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