Sizing silver nanoparticles in chicken meat using direct slurry sampling graphite furnace atomic absorption spectrometry.

Recently, graphite furnace atomic absorption spectrometry (GFAAS) has been suggested as a tool for detection and sizing of metal nanoparticles (NPs) providing several advantages, such as direct analysis of solid samples, high sample throughput, and robust and cost-efficient instrumentation. For this purpose, evaluation of newly introduced criteria of the absorbance signal, namely, atomization delay (tad) and atomization rate (kat), is performed. However, in real samples, NPs are typically stabilized by either engineered coating reagents or natural materials and occur in unknown concentration. Hence, systematic investigation of possible influences of nine different coating reagents and of Ag concentration on the atomization behavior of silver nanoparticles (AgNPs) was studied. Evaluation of absorption signal characteristics revealed no influence of the coating or Ag concentration on the observed parameters. Furthermore, size-dependent measurements gave reproducible size correlation independent from the coating. Validity of sizing AgNPs with the proposed approach was successfully proven by investigation of two reference materials. The found size of 74.3 ± 5.9 nm in RM 8017 (NIST) agrees very well with the certified size of 74.6 ± 3.8 nm. Moreover, AgNP size of 25.1 ± 2.5 nm found by direct slurry sampling GFAAS in matrix reference material "NanoLyse13"-chicken meat homogenate spiked with PVP-AgNPs-was in very good agreement with the reference value of 27.3 ± 5.3 nm as determined by TEM.