Bo Fu1,2, Anqi Zhang1, Mingli Li3, Li Pan1, Wenqiang Tang1, Meng An4, Wei Liu1, Jianqiong Zhang5. 1. Department of Central Laboratory, Liaocheng People's Hospital, Liaocheng, PR China. 2. School of Basic Medical Science, Shandong University, Jinan, PR China. 3. Department of Clinical Laboratory, Weifang Traditional Chinese Hospital, Weifang, PR China. 4. Department of Clinical Laboratory, Liaocheng People's Hospital, Liaocheng, PR China. 5. Jiangsu Key Laboratory of Molecule Imaging & Functional Imaging, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, PR China.
Abstract
AIM: To explore the circular RNA (circRNA) profile of breast cancer brain metastasis (BCBM). MATERIALS & METHODS: RNA-seq was performed to identify the circRNA expression profile of brain metastatic breast cancer cell line 231-BR, in comparison with its parental nonspecific metastatic cell line MDA-MB-231. RESULTS: A total of 215 upregulated and 191 downregulated circRNAs were identified. The expression levels of ten randomly selected majorly altered circRNAs were re-examined by real-time quantitative PCR and agarose gel electrophoresis, and the following alterations were verified: upregulation of hsa_circ_0001944, hsa_circ_0001481, hsa_circ_0000646, hsa_circ_0001006 and hsa_circ_0000732, and downregulation of hsa_circ_0001910, hsa_circ_0008285 and hsa_circ_0000002. CircRNA/miRNA analysis revealed that hsa_circ_0001944 may be involved in BCBM through sponging up miR-509 and interfering with its binding to the downstream targets. CONCLUSION: This study provides a leading and fundamental circRNA profile of BCBM.
AIM: To explore the circular RNA (circRNA) profile of breast cancer brain metastasis (BCBM). MATERIALS & METHODS: RNA-seq was performed to identify the circRNA expression profile of brain metastatic breast cancer cell line 231-BR, in comparison with its parental nonspecific metastatic cell line MDA-MB-231. RESULTS: A total of 215 upregulated and 191 downregulated circRNAs were identified. The expression levels of ten randomly selected majorly altered circRNAs were re-examined by real-time quantitative PCR and agarose gel electrophoresis, and the following alterations were verified: upregulation of hsa_circ_0001944, hsa_circ_0001481, hsa_circ_0000646, hsa_circ_0001006 and hsa_circ_0000732, and downregulation of hsa_circ_0001910, hsa_circ_0008285 and hsa_circ_0000002. CircRNA/miRNA analysis revealed that hsa_circ_0001944 may be involved in BCBM through sponging up miR-509 and interfering with its binding to the downstream targets. CONCLUSION: This study provides a leading and fundamental circRNA profile of BCBM.
Entities:
Keywords:
biomarker; breast cancer brain metastasis; circular RNA; expression profile; therapeutic target
Authors: K Li; Q Guo; X Zhang; X Dong; W Liu; A Zhang; Y Li; J Yan; G Jia; Z Zheng; W Tang; L Pan; M An; B Zhang; S Liu; B Fu Journal: Clin Exp Immunol Date: 2019-11-08 Impact factor: 4.330