Romain Kania1, Pierre Vironneau1, Huong Dang1, Béatrice Bercot2, Emmanuelle Cambau2, Benjamin Verillaud1, Domitille Camous1, Gerda Lamers3, Philippe Herman1, Eric Vicaut4, Natacha Tessier5, Thierry Van Den Abbeele5. 1. a Department of Otorhinolaryngology, Head & Neck Surgery , Lariboisière University Hospital, APHP, UMR 1141 Center for the Developing Brain, Paris Biobank BB-0033-00064, Platform of Biopathology and Innovative Technologies for Health, Paris 7 University, Paris Sorbonne Cité , Paris , France. 2. b Department of Bacteriology , Lariboisière University Hospital, APHP, Paris 7 University, Paris Sorbonne Cité , Paris , France. 3. c Institute of Biology Leiden, Leiden University , Leiden , The Netherlands. 4. d Clinical Research Unit Fernand Widal University Hospital, APHP, Paris 7 University, Paris Sorbonne Cité , Paris , France. 5. e Department of Pediatric Otorhinolaryngology , Head & Neck Surgery, Robert Debré University Hospital APHP, Paris 7 University, Sorbonne Cité , Paris , France.
Abstract
BACKGROUND: Biofilms are communities of bacteria embedded in a self-produced glycocalyx matrix. Adenoids have been shown to harbor bacterial biofilms. Aim/objectives: To compare the prevalence of biofilms in adenoid of children with chronic otitis media (COM) (group1) versus a control group without any COM (group 2) having adenoids removed because of hypertrophy. MATERIAL AND METHODS: One hundred and three children were prospectively enrolled in this case-control study, group 1 (n = 52) and group 2 (n = 51). The main outcome measurement was the prevalence of biofilm in adenoidectomy specimens analyzed using confocal laser scanning microscopy. Children in group 1 who had middle ear (ME) effusion and requiring the insertion of a tympanostomy tube underwent biopsy of the ME mucosa and effusion sampling. RESULTS: Biofilms were found in adenoids' specimens of both groups and in the ME biopsy and effusion. The biofilm prevalence in adenoids was 63.5% (33/52) in group 1 and 47.1% (24/51) in group 2. Day nursery and previous antibiotics intake were significantly more frequent in group 1 than in group 2. CONCLUSIONS AND SIGNIFICANCE: This case-control study demonstrates that adenoid tissue in children with COM contains more mucosal biofilms than adenoid tissue removed for hypertrophy. Biofilm was seen in ME biopsies and effusion.
BACKGROUND: Biofilms are communities of bacteria embedded in a self-produced glycocalyx matrix. Adenoids have been shown to harbor bacterial biofilms. Aim/objectives: To compare the prevalence of biofilms in adenoid of children with chronic otitis media (COM) (group1) versus a control group without any COM (group 2) having adenoids removed because of hypertrophy. MATERIAL AND METHODS: One hundred and three children were prospectively enrolled in this case-control study, group 1 (n = 52) and group 2 (n = 51). The main outcome measurement was the prevalence of biofilm in adenoidectomy specimens analyzed using confocal laser scanning microscopy. Children in group 1 who had middle ear (ME) effusion and requiring the insertion of a tympanostomy tube underwent biopsy of the ME mucosa and effusion sampling. RESULTS: Biofilms were found in adenoids' specimens of both groups and in the ME biopsy and effusion. The biofilm prevalence in adenoids was 63.5% (33/52) in group 1 and 47.1% (24/51) in group 2. Day nursery and previous antibiotics intake were significantly more frequent in group 1 than in group 2. CONCLUSIONS AND SIGNIFICANCE: This case-control study demonstrates that adenoid tissue in children with COM contains more mucosal biofilms than adenoid tissue removed for hypertrophy. Biofilm was seen in ME biopsies and effusion.
Entities:
Keywords:
Biofilms; adenoids; microbiota; otitis media
Authors: Jianbin Guo; Guihua Cao; Xing Wang; Wenhao Tang; Weilong Diwu; Ming Yan; Min Yang; Long Bi; Yisheng Han Journal: Int J Nanomedicine Date: 2021-10-27