Biserka Mulac-Jeričević1, Sandra Šućurović1, Tamara Gulic1, Julia Szekeres-Bartho2,3,4,5. 1. Department of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia. 2. Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, Pecs University, Pecs, Hungary. 3. János Szentágothai Research Centre, Pecs University, Pecs, Hungary. 4. Endocrine Studies, Centre of Excellence, Pecs University, Pecs, Hungary. 5. MTA - PTE Human Reproduction Research Group, Pecs, Hungary.
Abstract
PROBLEM: The progesterone-regulated genes, PIBF and Gal-1, are key players in the feto-maternal immunological interaction. This study aims to investigate the expression of PIBF and Gal-1 in WT and progesterone receptor KO models as well as subsequent effects of PIBF on decidualization of stromal cells. METHOD OF THE STUDY: PRAKO, PRBKO and PRKO BALB/c mice were used for assessing the role of PR isoforms in PIBF induction. PIBF- and Gal-1 mRNA expression in the uterus was tested by real-time PCR. The effect of PIBF on decidualization of endometrial stromal cells was verified by anti-desmin immunofluorescence. Immunohistochemistry was used for testing PIBF expression in the uterus. Gal-1, ERα and PR positive decidual NK cells were detected by immunofluorescence. RESULTS: PIBF mRNA was significantly increased in progesterone-treated WT mice, but not in PRKO and PRAKO mice. PIBF protein expression was reduced in the endometria of PRKO and PRAKO, but not in PRBKO mice. During a 6-day culture, PIBF induced decidual transformation of endometrial stromal cells. PIBF expression in the mouse uterus was highest during the implantation window, while Gal-1 mRNA expression continuously increased between day 2.5 and day 11.5 of gestation. Decidual NK cells express Gal-1 and ERα, but not PR at day 7.5 murine pregnancy. CONCLUSION: PIBF produced via engagement of PRA, is highly expressed in the endometrium during the implantation window, and plays a role in decidualization. The concerted action of PIBF and Gal-1 might contribute to the low cytotoxic activity of decidual NK cells.
PROBLEM: The progesterone-regulated genes, PIBF and Gal-1, are key players in the feto-maternal immunological interaction. This study aims to investigate the expression of PIBF and Gal-1 in WT and progesterone receptor KO models as well as subsequent effects of PIBF on decidualization of stromal cells. METHOD OF THE STUDY: PRAKO, PRBKO and PRKO BALB/c mice were used for assessing the role of PR isoforms in PIBF induction. PIBF- and Gal-1 mRNA expression in the uterus was tested by real-time PCR. The effect of PIBF on decidualization of endometrial stromal cells was verified by anti-desmin immunofluorescence. Immunohistochemistry was used for testing PIBF expression in the uterus. Gal-1, ERα and PR positive decidual NK cells were detected by immunofluorescence. RESULTS: PIBF mRNA was significantly increased in progesterone-treated WT mice, but not in PRKO and PRAKO mice. PIBF protein expression was reduced in the endometria of PRKO and PRAKO, but not in PRBKO mice. During a 6-day culture, PIBF induced decidual transformation of endometrial stromal cells. PIBF expression in the mouse uterus was highest during the implantation window, while Gal-1 mRNA expression continuously increased between day 2.5 and day 11.5 of gestation. Decidual NK cells express Gal-1 and ERα, but not PR at day 7.5 murine pregnancy. CONCLUSION: PIBF produced via engagement of PRA, is highly expressed in the endometrium during the implantation window, and plays a role in decidualization. The concerted action of PIBF and Gal-1 might contribute to the low cytotoxic activity of decidual NK cells.
Authors: Timea Csabai; Eva Pallinger; Arpad F Kovacs; Eva Miko; Zoltan Bognar; Julia Szekeres-Bartho Journal: Front Immunol Date: 2020-03-11 Impact factor: 7.561