Jaqueline Scapinello1, Liz Girardi Müller2, Monica S Z Schindler2, Gabriela Sabrina Anzollin2, Anna Maria Siebel2, Aline A Boligon3, Rivaldo Niero4, Thalia E S Saraiva5, Nathália Pulz Maus5, Andresa Heemann Betti5, J Vladimir Oliveira6, Jacir Dal Magro2, Débora de Oliveira6. 1. Department of Chemical and Food Engineering, UFSC, Florianópolis 88040-900, SC, Brazil; Area of Exact and Environmental Sciences and Area of Health Sciences, Universidade Comunitária da Região de Chapecó (Unochapecó), Chapecó, SC, Brazil. Electronic address: jaquescapinello@gmail.com. 2. Area of Exact and Environmental Sciences and Area of Health Sciences, Universidade Comunitária da Região de Chapecó (Unochapecó), Chapecó, SC, Brazil. 3. Center of Health Sciences, UFSM, Santa Maria, RS, Brazil. 4. Department of Biological Sciences, Univali, Itajaí, SC, Brazil. 5. Institute of Health Sciences, Universidade Feevale, Novo Hamburgo, RS, Brazil. 6. Department of Chemical and Food Engineering, UFSC, Florianópolis 88040-900, SC, Brazil.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Popular medicine use stems of Philodendron bipinnatifidum (Araceae) in inflammation cases, such as in erysipelas, as well as orchitis and rheumatism treatment. The present study, conducted for the first time in literature, investigate the antinociceptive and anti-inflammatory activities of P. bipinnatifidum stems ethyl acetate extract (EPB). MATERIALS AND METHODS: GC/MS and HPLC analysis were performed for EPB extract. We used EPB at 250, 375 and 500 mg/kg (oral route, p.o.) in male Swiss mice. The antinociceptive activity of the plant extract assessed by acetic acid induced writhing and formalin tests. To investigate the possible participation of opioid system in EPB-mediated effects, we previously administered naloxone to the mice. Anti-inflammatory activity was evaluated using carrageenan-induced paw oedema. The open-field test aimed to investigate the possible EPB effects on the locomotor and exploratory activities. To assess the protective role of EPB on carrageenan-induced oxidative stress, the levels of NPSH, TBARS, as well as SOD and CAT activities were evaluated in blood and paw tissue. The acute toxicity of the EPB was investigated using OECD 423 guideline. RESULTS: The EPB chemical analysis by GC/MS and HPLC revealed the presence of flavonoids (luteolin and quercetin) and phytosterols (β-sitosterol and stigmasterol). The oral treatment with the EPB inhibited mice abdominal writhings (P < 0.01) at 375 and 500 mg/kg, and reduced the formalin effect at the first-phase (500 mg/kg, P < 0.05) and also at the second-phase (500 mg/kg, P < 0.001) of the test. EPB (375 and 500 mg/kg) did not alter spontaneous locomotion in open field test, however the number of fecal bolus was significantly lower for the EPB group at 500 mg/kg when compared to the vehicle group (P < 0.05). The pretreatment with naloxone caused significant inhibition of antinociceptive activity induced by EPB in the formalin test, revealing the possible involvement of opioid receptors. EPB extract administered at 500 mg/kg (p.o.) prevented carrageenan-induced paw oedema (P < 0.05 and 0.01) until 6 h after carragenan injection. Evaluation of TBARS and NPSH levels, SOD and CAT activities in the blood and paw tissue of animals submitted to the carrageenan assay suggested that the anti-inflammatory effect of EPB may be linked to oxidative stress inhibition. The acute administration of the EPB (2000 mg/kg, p.o.) caused no mortality, demonstrating low toxicity. CONCLUSIONS: The extract of P. bipinnatifidum displays antinociceptive and anti-inflammatory activities, causing no toxicological effects. The pharmacological activity of this vegetal species may be related to the presence of flavonoids and phytosterols. Our results support the ethnomedical use of this vegetal species as analgesic and anti-inflammatory agent.
ETHNOPHARMACOLOGICAL RELEVANCE: Popular medicine use stems of Philodendron bipinnatifidum (Araceae) in inflammation cases, such as in erysipelas, as well as orchitis and rheumatism treatment. The present study, conducted for the first time in literature, investigate the antinociceptive and anti-inflammatory activities of P. bipinnatifidum stems ethyl acetate extract (EPB). MATERIALS AND METHODS: GC/MS and HPLC analysis were performed for EPB extract. We used EPB at 250, 375 and 500 mg/kg (oral route, p.o.) in male Swiss mice. The antinociceptive activity of the plant extract assessed by acetic acid induced writhing and formalin tests. To investigate the possible participation of opioid system in EPB-mediated effects, we previously administered naloxone to the mice. Anti-inflammatory activity was evaluated using carrageenan-induced paw oedema. The open-field test aimed to investigate the possible EPB effects on the locomotor and exploratory activities. To assess the protective role of EPB on carrageenan-induced oxidative stress, the levels of NPSH, TBARS, as well as SOD and CAT activities were evaluated in blood and paw tissue. The acute toxicity of the EPB was investigated using OECD 423 guideline. RESULTS: The EPB chemical analysis by GC/MS and HPLC revealed the presence of flavonoids (luteolin and quercetin) and phytosterols (β-sitosterol and stigmasterol). The oral treatment with the EPB inhibited mice abdominal writhings (P < 0.01) at 375 and 500 mg/kg, and reduced the formalin effect at the first-phase (500 mg/kg, P < 0.05) and also at the second-phase (500 mg/kg, P < 0.001) of the test. EPB (375 and 500 mg/kg) did not alter spontaneous locomotion in open field test, however the number of fecal bolus was significantly lower for the EPB group at 500 mg/kg when compared to the vehicle group (P < 0.05). The pretreatment with naloxone caused significant inhibition of antinociceptive activity induced by EPB in the formalin test, revealing the possible involvement of opioid receptors. EPB extract administered at 500 mg/kg (p.o.) prevented carrageenan-induced paw oedema (P < 0.05 and 0.01) until 6 h after carragenan injection. Evaluation of TBARS and NPSH levels, SOD and CAT activities in the blood and paw tissue of animals submitted to the carrageenan assay suggested that the anti-inflammatory effect of EPB may be linked to oxidative stress inhibition. The acute administration of the EPB (2000 mg/kg, p.o.) caused no mortality, demonstrating low toxicity. CONCLUSIONS: The extract of P. bipinnatifidum displays antinociceptive and anti-inflammatory activities, causing no toxicological effects. The pharmacological activity of this vegetal species may be related to the presence of flavonoids and phytosterols. Our results support the ethnomedical use of this vegetal species as analgesic and anti-inflammatory agent.
Authors: Alessandro R Paulazzi; Bianca O Alves; Gabriela A L Zilli; Aline E Dos Santos; Fernanda Petry; Krissie D Soares; Letícia J Danielli; Jefferson Pedroso; Miriam A Apel; Gean Pablo S Aguiar; Anna M Siebel; J Vladimir Oliveira; Liz Girardi Müller Journal: Inflammopharmacology Date: 2022-01-10 Impact factor: 4.473
Authors: Maryelen A Souza; Jaqueline Scapinello; João G G Guzatti; Mikaela Scatolin; Rafael Martello; Monica S Z Schindler; Jean F F Calisto; Bianca Alves; Letícia V Morgan; J Vladimir Oliveira; Jacir Dal Magro; Liz G Müller Journal: Biomed J Date: 2020-07-02 Impact factor: 7.892
Authors: Fernanda Petry; Bruna B Dall'Orsoleta; Mikaela Scatolin; Leticia V Morgan; Bianca O Alves; Gabriela S Anzollin; Gabriela A L Zilli; Jaqueline Scapinello; Leticia J Danielli; Krissie D Soares; Miriam Apel; J Vladimir Oliveira; Jacir Dal Magro; Liz G Müller Journal: Evid Based Complement Alternat Med Date: 2021-02-26 Impact factor: 2.629