| Literature DB >> 30798547 |
Shweta Fulsundar1, Sara Domingues2, Kaare Magne Nielsen3.
Abstract
The role of vesicle-mediated gene transfer in Acinetobacter baumannii populations has been investigated in the last decade. Importantly, outer membrane vesicles (OMVs) secreted from A. baumannii cells have proven to be efficient agents of transfer of antimicrobial resistance genes to other bacterial species. However, the measurement of vesicle-mediated transfer depends on many experimental parameters. Here, we describe an experimental method useful to study transfer of DNA via membrane vesicles of A. baumannii in various bacterial populations.Entities:
Keywords: Acinetobacter baumannii; Antimicrobial resistance; Horizontal gene transfer; Outer membrane vesicle; Transfer frequency
Mesh:
Year: 2019 PMID: 30798547 DOI: 10.1007/978-1-4939-9118-1_9
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745