| Literature DB >> 30796820 |
Shashi Bhushan Chauhan1, Rebecca Faleiro2,3, Rajiv Kumar4,5, Susanna Ng2, Bhawana Singh1, Om Prakash Singh1, Siddharth Sankar Singh1, Fiona Amante2, Fabian de Labastida Rivera2, Madhukar Rai1, Jaya Chakravarty1, David Sacks6, Susanne Nylen7, Shyam Sundar1, Christian Engwerda2.
Abstract
Control of visceral leishmaniasis (VL) caused by Leishmania donovani requires interferon-γ production by CD4+ T cells. In VL patients, antiparasitic CD4+ T-cell responses are ineffective for unknown reasons. In this study, we measured the expression of genes associated with various immune functions in these cells from VL patients and compared them to CD4+ T cells from the same patients after drug treatment and from endemic controls. We found reduced GATA3, RORC, and FOXP3 gene expression in CD4+ T cells of VL patients, associated with reduced Th2, Th17, and FOXP3+CD4+ T regulatory cell frequencies in VL patient blood. Interleukin 2 (IL-2) was an important upstream regulator of CD4+ T cells from VL patients, and functional studies demonstrated the therapeutic potential of IL-2 for improving antiparasitic immunity. Together, these results provide new insights into the characteristics of CD4+ T cells from VL patients that can be used to improve antiparasitic immune responses.Entities:
Keywords: zzm321990 Leishmania donovanizzm321990 ; CD4+ T cells; IL-2; visceral leishmaniasis
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Year: 2019 PMID： 30796820 PMCID： PMC6775044 DOI： 10.1093/infdis/jiz074
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226