| Literature DB >> 30788819 |
Tennille Sibbritt1,2, Pierre Osteil1,2, Xiaochen Fan1, Jane Sun1, Nazmus Salehin1, Hilary Knowles1, Joanne Shen1, Patrick P L Tam3,4.
Abstract
Efficient and reliable methods for gene editing are critical for the generation of loss-of-gene function stem cells and genetically modified mice. Here, we outline the application of CRISPR-Cas9 technology for gene editing in mouse embryonic stem cells (mESCs) to generate knockout ESC chimeras for the fast-tracked analysis of gene function. Furthermore, we describe the application of gene editing directly to mouse epiblast stem cells (mEpiSCs) for modelling germ layer differentiation in vitro.Entities:
Keywords: CRISPR-Cas9; Embryonic stem cells; Epiblast stem cells
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Year: 2019 PMID： 30788819 DOI： 10.1007/978-1-4939-9086-3_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745