| Literature DB >> 30788817 |
Michael R Larcombe1,2,3, Jan Manent1,2,3, Joseph Chen1,2,3, Ketan Mishra1,2,3, Xiaodong Liu1,2,3, Christian M Nefzger4,5,6,7.
Abstract
Lentiviral gene transfer technologies exploit the natural efficiency of viral transduction to integrate exogenous genes into mammalian cells. This provides a simple research tool for inducing transgene expression or endogenous gene knockdown in both dividing and nondividing cells. This chapter describes an improved protocol for polyethylenimine (PEI)-mediated multi-plasmid transfection and polyethylene glycol (PEG) precipitation to generate and concentrate lentiviral vectors.Entities:
Keywords: Gene transfer; PEG precipitation; PEI transfection; Titration
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Year: 2019 PMID： 30788817 DOI： 10.1007/978-1-4939-9086-3_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745