Bone microarchitecture and bone turnover in hepatic cirrhosis.

Liver cirrhosis leads to bone loss. To date, information on bone quality (three-dimensional microarchitecture) and, thus, bone strength is scarce. We observed decreased bone quality at both assessed sites, independent of disease severity. Therefore, all patients should undergo early-stage screening for osteoporosis.
INTRODUCTION: Recent studies found low bone mineral density in cirrhosis, but data on bone microstructure are scarce. This study assessed weight-bearing and non-weight-bearing bones in patients with cirrhosis and healthy controls. The primary objective was to evaluate trabecular and cortical microarchitecture.
METHODS: This was a single-center study in patients with recently diagnosed hepatic cirrhosis. Thirty-two patients and 32 controls participated in this study. After determining the type of cirrhosis, the parameters of bone microarchitecture were assessed by high-resolution peripheral quantitative computed tomography.
RESULTS: Both cortical and trabecular microarchitectures showed significant alterations. At the radius, trabecular bone volume fraction was 17% lower (corrected p = 0.028), and, at the tibia, differences were slightly more pronounced. Trabecular bone volume fraction was 19% lower (p = 0.024), cortical bone mineral density 7% (p = 0.007), and cortical thickness 28% (p = 0.001), while cortical porosity was 32% higher (p = 0.023), compared to controls. Areal bone mineral density was lower (lumbar spine - 13%, total hip - 11%, total body - 9%, radius - 17%, and calcaneus - 26%). There was no correlation between disease severity and microarchitecture. Areal bone mineral density (aBMD) measured by dual-energy X-ray absorptiometry (DXA) correlated well with parameters of cortical and trabecular microarchitecture.
CONCLUSIONS: Hepatic cirrhosis deteriorates both trabecular and cortical microarchitecture, regardless of disease severity. Areal bone mineral density is diminished at all sites as a sign of generalized affection. In patients with hepatic cirrhosis, regardless of its origin or disease severity, aBMD measurements are an appropriate tool for osteologic screening.

Introduction

Chronic liver disease is a common medical issue with a globally rising prevalence and high risk of cirrhosis. In hepatic cirrhosis, pathological metabolic changes result in various comorbidities, including a higher risk of osteoporosis [1].

Previous studies have found decreased areal bone mineral density (aBMD) at the lumbar spine [2, 3]. At the hip, no significant differences were observed as compared with controls [2-4]. However, even though dual X-ray absorptiometry (DXA) is widely used to assess fracture risk, the assessment of aBMD alone has some limitations. For instance, in a study in patients with diabetes, aBMD alone was unable to explain the increased fracture risk, and, therefore, adding important information by assessing bone microarchitecture clearly improved the diagnostic value in this investigation [5]. Still, high-resolution peripheral quantitative computed tomography (HR-pQCT) remains not systematically used in clinical routine.

To date, the literature on bone microarchitecture in liver cirrhosis has remained scarce. Several investigations have addressed histomorphometric evaluation in chronic liver disease, but the studies were inconsistent concerning etiology and stages of hepatic disease. In addition, use of control groups has been infrequent. Only two studies evaluated patients with cirrhosis. Trabecular bone volume fraction (Tb.BV/TV) was reduced [6, 7], although other studies observed normal values in chronic liver disease. Likewise, comments on detailed trabecular microarchitecture remain rare. One study [6] observed decreased trabecular thickness (Tb.Th) in cirrhosis. Information on cortical bone in patients with cirrhosis is scarce, as well. Cortical thickness (Ct.Th) was decreased [6], and cortical porosity (Ct.Po) has not yet been evaluated.

Histomorphometry is associated with several disadvantages. First, biopsies need to be taken; an invasive procedure with such potential complications as fractures or infections. Second, the biopsies are mostly performed at the iliac crest. Therefore, this method provides no reliable information on the microarchitecture of other skeletal regions, such as weight-bearing long bones, as their structure may clearly differ from that at the ilium. Third, thin samples of static histomorphometry taken from large specimens are limited to a two-dimensional (2D) view. Values of 2D histomorphometry do not consistently correlate with those measured by three-dimensional (3D) radiological methods, as the microarchitecture at the iliac crest shows high inter-individual variability and 2D histomorphometry is limited to distinct histological sections, thereby only providing a glimpse of a small part of the bone [8].

Through the use of non-invasive 3D HR-pQCT [9], investigators can assess both weight-bearing (distal tibia) and non-weight-bearing (distal radius) bones in vivo and, thus, examine cortical and trabecular microarchitecture independently from one another. HR-pQCT results correlate strongly with both microarchitecture and failure loads at the lumbar spine and proximal femur [10]. To date, 3D bone microarchitecture has not been explored in hepatic cirrhosis.

The primary objective of this study was to evaluate trabecular and cortical bone microarchitecture, assessed by HR-pQCT, in patients with hepatic cirrhosis compared with age- and sex-matched healthy controls.

Our secondary objectives were to evaluate aBMD at the lumbar spine, hip, total body, and calcaneus, trabecular bone score values, and serum markers of bone turnover.

Subjects and methods

Subjects

This single-center study was conducted at the Medical Department II of St. Vincent Hospital, an academic teaching hospital of the Medical University of Vienna and a referral center for gastrointestinal, metabolic, and bone diseases in Vienna, Austria. Female and male patients with recently diagnosed hepatic cirrhosis, who were admitted to this center to determine the type of cirrhosis and initiate liver-specific therapy, were screened for eligibility.

This study was approved by the Local Ethics Committee (Approval no. 003-02-2013), and the participants’ written informed consent was obtained prior to any patient-related procedures. This study was conducted in accordance with the Declaration of Helsinki.

The inclusion criteria were diagnosis of liver cirrhosis based on clinical presentation, medical history, imaging (sonography, computed tomography [CT] scan, or magnetic resonance imaging), and laboratory parameters, in line with the current guidelines for the diagnosis of hepatic disorders [11, 12]. In case of uncertainty, a biopsy was performed.

The etiologies of cirrhosis were the following: (1) alcoholic liver disease, defined as daily ethanol ingestion greater than 40 g in men and 20 g in women over the last years and no other causes for cirrhosis; (2) chronic viral hepatitis B or C, based on positive polymerase chain reaction results for the respective virus; (3) autoimmune hepatitis, based on a score equal or greater than 6 in the simplified autoimmune hepatitis score and responsiveness to corticosteroids; (4) non-alcoholic fatty liver disease, defined as the presence of hepatic steatosis detected by histology and no cause for secondary hepatic fat accumulation, such as significant alcohol consumption, use of steatogenic medication, or hereditary disorders; and (5) storage diseases, such as hemochromatosis based on histology and typical laboratory findings. Six patients with primary biliary cirrhosis or primary sclerosing cholangitis were screened but excluded, as they did not fulfill the eligibility criteria concerning comorbidities.

The exclusion criteria were age < 18 years; pregnancy or lactation; history of high-energy trauma; gastrointestinal and endocrine diseases (inflammatory bowel disease, i.e., Crohn’s disease, ulcerative colitis); chronic kidney disease (chronic kidney disease stages II–V, renal association); insulin-dependent diabetes mellitus type I or II; hypo- or hyperthyroid metabolic status (substitution was not an exclusion criterion); biliary obstruction; oncological and rheumatic diseases (rheumatoid arthritis, spondyloarthritis, systemic lupus erythematosus); congestive heart failure (New York Heart Association Classes II–IV); human immunodeficiency virus infection; drug abuse; immobility, wheelchair dependence, or bedridden state; body mass index < 20 and > 35 kg/m2; and treatment with the following medications: any prior liver-specific medication (except nutrition supplements, e.g., silibinin), any medication that could cause elevation of liver enzymes (e.g., methotrexate, chronic paracetamol use), adrenal or anabolic steroids (e.g., > 5 mg prednisolone per day), glitazones, anticonvulsants, long-term use of anticoagulants (> 10 years), and/or any osteoporosis treatment (including calcium and/or vitamin D supplementation).

The healthy controls (subject to the same exclusion criteria but no history and no laboratory evidence of liver disease) were recruited from active and retired hospital staff.

Radiology

Vertebral fractures were detected by digital radiographs according to the Genant criteria. The patients received an abdominal ultrasound to assess ascites and to exclude malignancies.

Two technologists certified by the International Osteoporosis Foundation/International Society for Clinical Densitometry applied DXA to assess aBMD.

The lumbar spine (L1–L4), non-dominant radius (except for previous fracture), total body, total hip, and femoral neck were scanned after daily calibration with standardized phantoms (iDXA®, GE Healthcare Lunar, Madison, WI, USA, latest software version). Fractured vertebrae were excluded from all investigations. The in vivo precision of error, expressed as coefficient of variation (CV), was 0.41% for the lumbar spine and 0.53% for the hip [13].

In addition, in a subgroup of 19 patients and 28 controls, trabecular bone score was automatically assessed from lumbar DXA via TBS iNsight® (Medimaps SA, Canéjan, France, latest software version). In 9 patients and 8 controls, calcaneal BMD was assessed by dual X-ray and laser (DXL; Calcscan®, Demtech AB, Taby, Sweden, latest software version). According to the literature, the CV was 1.19% [14]. Due to technical issues (a deletion of raw data at the DXA device due to a software update, technical failure of DXL over a period of several months, and lack of technical support by the Swedish hardware provider), we were unable to obtain the trabecular bone score and calcaneal BMD from all subjects. These subjects were not selected deliberately.

Bone microarchitecture

Two specifically trained technologists performed the HR-pQCT examinations (XtremeCT, Scanco Medical, Brütisellen, Switzerland) following daily calibrations with a standardized phantom (Moehrendorf, Germany) and according to the manufacturer’s standard in vivo protocol. The non-dominant (except for previous fracture) distal radius and distal tibia were scanned while immobilized in a carbon-fiber cast. The manufacturer’s standard software was used for all measurements and evaluations, and the reference lines were set manually according to the anteroposterior scout view. There were 110 CT slices (82 μm voxel size), with the first slice 9.5 mm proximal to the reference line. Each measurement lasted 2.8 min, and the effective dose was lower than 3 μSv. The Scanco SOP scale was used to exclude scans with motion artifacts graded higher than 3.

The following parameters of volumetric BMD (vBMD), bone microstructure, and bone geometry were assessed: cortical BMD, Tb.BV/TV, trabecular number (Tb.N/mm), Tb.Th, trabecular separation (Tb.Sp), Ct.Th, and cortical porosity (Ct.Po).

Clinical chemistry and serum markers of bone turnover

Parameters of bone turnover were obtained after overnight fasting (sampling between 8 and 10 a.m.) at the ISO 9001-certified laboratory (Labcon Ltd) at the St. Vincent Hospital. The following parameters were determined: calcium, alkaline phosphatase, phosphorus, C-terminal telopeptide of type I collagen (CTX), 25-OH vitamin D, intact parathyroid hormone (iPTH), and procollagen type 1 N-terminal peptide (P1NP). Furthermore, the following parameters related to liver disease were determined: aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, creatinine, albumin, bilirubin, international normalized ratio, and partial thromboplastin time (IDS-iSYS microparticle immunoassay system, Immunodiagnostics Systems Ltd., Boldon, UK; Architect ci8200 platform, Abbott Laboratories, Abbott Park, IL, USA; and ACL TOP 500 CTS, Instrumentation Laboratory, Bedford, MA, USA). Serum calcium was adjusted for low albumin.

Statistical analysis

Sample size calculations are based on standard deviations from a pilot evaluation of Tb.BV/TV in 8 patients with cirrhosis (3 due to alcoholic liver disease, 3 due to viral hepatitis, and 2 due to non-alcoholic fatty liver disease). In order to detect a mean difference in Tb.BV/TV of 0.03 with a power of 80%, 32 patients were to be included per group.

Central tendency and dispersion measurements were calculated for all variables. Group differences between patients and controls and between patients with alcoholic liver disease and non-alcoholic etiology were carried out using two-sample t-tests. Distributional assumptions for t-tests were checked visually by quantile–quantile plots.

Multiple linear regression models were performed to evaluate the relationship between cortical and trabecular microarchitecture parameters and aBMD measures, serum markers of bone turnover (alkaline phosphatase, iPTH, and CTX), and Child-Pugh and Model for End-Stage Liver Disease (MELD) scores. Separate models were derived for each microarchitecture parameter as dependent variable and BMD or serum markers of bone turnover measures as explanatory variables. Model fits were quantified by R2 values and p values. For each model, the improvement in model fit by MELD and Child-Pugh scores was tested using F-tests.

All tests were two-sided, and p values less than 0.05 were considered statistically significant. As bone microarchitecture is described by multiple parameters, corresponding p values were adjusted to control a false discovery rate (FDR) of 0.05. All statistical analyses were performed with the statistical software R version 3.50 (R Development Core Team, 2018).

Results

Study demographics

In total, 32 patients (12 women) and 32 healthy age- and sex-matched controls (12 women) with a median age of 62 and 60 years, respectively, were included—16 patients with alcoholic liver disease and 16 with non-alcoholic etiology (8 with viral cirrhosis, 5 with non-alcoholic fatty liver disease, 2 with hemochromatosis, and one with autoimmune hepatitis). The demographic data were similar between patients and healthy controls with the exception of higher alcohol intake in patients (Table 1).

Table 1

Demographic data as median with interquartile range (Q1–Q3). p values are given for patients compared with controls (cirrhosis vs. controls). There were no differences between alcoholic cirrhosis and non-alcoholic cirrhosis except higher alcohol consumption (p = 0.01) and packyears of smoking (p = 0.01) in patients with alcoholic liver disease. MELD, model for end-stage liver disease

	Controls median values (Q1–Q3)	Cirrhosis median values (Q1–Q3)	Alcoholic liver disease median values (Q1–Q3)	Non-alcoholic median values (Q1–Q3)	p value cirrhosis vs. controls
Number of patients (n)	32	32	16	16
Age (years)	60 (54–67)	62 (57–68)	63 (59–69)	62 (56–65)	0.64
Body mass index	26.8 (24.5–30.2)	26.2 (23.5–31.0)	25.4 (23.4–28.2)	28.2 (24.5–31.5)	0.91
Child-Pugh score		6 (5–7)	5 (5–7)	6 (5–7)
MELD score		9 (7–12)	8 (6.8–11.3)	10.5 (8–12.5)
Packyears of smoking	5 (0–35)	27 (0–36)	33 (24–53)	17 (0–30)	0.22
Alcohol (units/d)	1 (0–1)	4 (0–10)	8 (4–13)	0 (0–3)	0.001

The Child-Pugh and MELD scores were similar between the groups; yet, the packyears of smoking and alcohol intake were higher among subjects with alcoholic liver disease.

Spinal X-ray identified low-trauma vertebral fractures in 13 patients with cirrhosis (40%), all of whom were asymptomatic (morphometric). Of these patients, five also had a history of at least one low-trauma non-vertebral fracture. None of the controls had a prevalent low-trauma vertebral or non-vertebral fracture.

At the radius, the patients and controls had similar cortical BMD values. In addition, no differences in Ct.Th or Ct.Po were found. However, Tb.BV/TV was significantly lower in cirrhotic patients, and Tb.Th only showed a trend to a decrease.

Alterations were more pronounced at the tibia. Among the patients, cortical BMD and Ct.Th were significantly lower with increased Ct.Po. Tb.BV/TV was significantly lower in the patients, as well, but there was only a trend towards higher Tb.Sp and lower Tb.Th and Tb.N (Table 2 and Fig. 1).

Table 2

Cortical and trabecular volumetric bone mineral density and bone microstructure of the radius and tibia. p values (cirrhosis vs. controls) are provided both corrected according to the false discovery rate adjustment and uncorrected. There were no differences between alcoholic cirrhosis and non-alcoholic cirrhosis. BMD, bone mineral density; Tb.BV/TV, trabecular bone volume fraction; Tb.N, trabecular number; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation; Ct.Th, cortical thickness; Ct.Po, cortical porosity

	Controls median values (Q1–Q3)	Cirrhosis median values (Q1–Q3)	Alcoholic liver disease median values (Q1–Q3)	Non-alcoholic median values (Q1–Q3)	Corrected p value cirrhosis vs. controls
Volumetric BMD
Radius cortical BMD (mg/cm3)	855 (811–887)	837 (802–892)	855 (797–888)	828 (805–900)	0.165
Tibia cortical BMD (mg/cm3)	843 (815–870)	780 (740–812)	779 (707–806)	783 (759–857)	0.007
Trabecular microstructure
Radius Tb. BV/TV	0.152 (0.137–0.174)	0.126 (0.096–0.154)	0.1335 (0.096–0.155)	0.123 (0.099–0.151)	0.028
Radius Tb.N (1/mm)	2.08 (1.89–2.17)	1.94 (1.7–2.17)	1.97 (1.29–2.19)	1.89 (1.74–2.17)	0.102
Radius Tb.Th (mm)	0.074 (0.065–0.084)	0.067 (0.058–0.076)	0.07 (0.059–0.077)	0.065 (0.058–0.076)	0.067
Radius Tb.Sp (mm)	0.402 (0.377–0.456)	0.45 (0.393–0.535)	0.446 (0.393–0.703)	0.455 (0.393–0.516)	0.077
Tibia BV/TV	0.168 (0.15–0.186)	0.136 (0.11–0.154)	0.13 (0.109–0.16)	0.141 (0.119–0.145)	0.024
Tibia Tb.N (1/mm)	2.13 (1.89–2.43)	1.94 (1.67–2.24)	2.02 (1.61–2.25)	1.9 (1.73–2.18)	0.067
Tibia Tb.Th (mm)	0.075 (0.07–0.084)	0.071 (0.061–0.078)	0.07 (0.056–0.075)	0.073 (0.065–0.08)	0.067
Tibia Tb.Sp (mm)	0.391 (0.332–0.442)	0.446 (0.379–0.521)	0.416 (0.379–0.547)	0.464 (0.389–0.511)	0.056
Cortical microstructure
Radius Ct.Th (mm)	0.79 (0.67–0.91)	0.68 (0.538–0.863)	0.79 (0.61–0.86)	0.63 (0.53–0.89)	0.15
Radius Ct.Po (%)	2.77 (2.26–3.69)	3.27 (2.15–4.75)	3.72 (2.78–4.8)	2.76 (1.98–4.03)	0.102
Tibia Ct.Th (mm)	1.17 (0.95–1.35)	0.84 (0.623–1.05)	0.86 (0.55–1.06)	0.81 (0.69–0.1)	0.001
Tibia Ct.Po (%)	7.96 (6.1–8.95)	10.55 (7.93–13.76)	11.79 (9.7–14.71)	9.36 (7.32–11.16)	0.023

Fig. 1

Images of a patient with hepatic cirrhosis, compared with a matched healthy control. a Sample of the distal radius; b sample of the distal tibia; c three-dimensional reconstruction of the distal radius; d three-dimensional reconstruction of the distal tibia; e three-dimensional visualization of cortical porosity. The images demonstrate both trabecular thinning and rarefication (higher separation between trabeculae), cortical thinning, as well as higher cortical porosity

Between the patients with and without low-trauma vertebral fractures, no differences in trabecular microarchitecture were observed. However, cortical BMD was lower at the tibia (759 vs. 805 mg/cm3, uncorrected p = 0.03), and there was a trend towards higher Ct.Po at the tibia (10.84 vs. 9.09%, uncorrected p = 0.06) in patients with low-trauma vertebral fractures.

Areal BMD and trabecular bone score

Areal BMD was lower in patients at all skeletal sites (lumbar spine, total hip, femoral neck, total body BMD, radius, and calcaneus). At both the lumbar spine and the hip, the Z scores were significantly lower in the patients compared with the controls: the difference was 1.25 at the lumbar spine and 0.85 at the hip.

Changes at the total hip and femoral neck were similar (Table 3).

Table 3

Areal bone mineral density of the lumbar spine, trabecular bone score, and areal bone mineral density of the total hip, femoral neck, total body, radius, and calcaneus. There were no differences between alcoholic and non-alcoholic cirrhosis

	Controls median values (Q1–Q3)	Cirrhosis median values (Q1–Q3)	Alcoholic liver disease median values (Q1–Q3)	Non-alcoholic median values (Q1–Q3)	p value cirrhosis vs. controls
Lumbar spine-BMD (L1–4. g/cm2)	1.123 (1.039–1.252)	0.981 (0.821–1.066)	0.98 (0.772–1.079)	0.984 (0.861–1.026)	0.001
T-score lumbar spine	− 0.45 (− 1.35–0.625)	− 1.75 (− 2.725 to − 0.75)	− 1.95 (− 3.63 to − 0.88)	− 1.6 (− 2.63 to − 0.48)	0.004
Z-score lumbar spine	− 0.05 (− 1.18–0.83)	− 1.3 (− 2–0.08)	− 1.65 (− 2.4–0.05)	− 0.95 (− 1.63–0.08)	0.034
Trabecular bone score	1.297 (1.172–1.389)	1.106 (1.034–1.235)	1.109 (1.061–1.259)	1.049 (1.009–1.229)	0.011
Total hip BMD (g/cm2)	1.023 (0.958–1.198)	0.908 (0.796–1.031)	0.862 (0.67–1.064)	0.924 (0.834–0.957)	0.006
T-score hip	− 0.1 (− 0.725–0.4)	− 1.1 (− 2.1 to − 0.275)	− 1.55 (− 2.75–0.3)	− 1.05 (− 1.38 to − 0.45)	0.005
Z-score hip	0.45 (− 0.1–0.8)	− 0.4 (− 1.38–0.33)	− 0.75 (− 2.2–0.9)	− 0.4 (− 0.5–0.3)	0.012
Femoral neck BMD (g/cm2)	0.895 (0.85–1.03	0.77 (0.66–0.85)	0.77 (0.58–0.89)	0.80 (0.74–0.83)	0.012
T-score femoral neck	− 0.65 (− 1.25–0.08)	− 1.4 (− 2.5 to − 0.78)	− 2.3 (− 3 to − 0.8)	− 0.9 (− 1.4 to − 0.8)	0.041
Z score femoral neck	0.15 (− 0.33–0.78)	− 0.85 (− 1.65–0.2)	− 1.3 (− 2.3–0.1)	− 0.2 (− 0.65–0.2)	0.037
Total body BMD (g/cm2)	1.224 (1.133–1.304)	1.118 (0.951–1.191)	1.124 (0.917–1.189)	1.118 (0.999–1.195)	0.003
Radius BMD (g/cm2)	0.736 (0.643–0.808)	0.611 (0.553–0.701)	0.656 (0.578–0.737)	0.57 (0.535–0.631)	0.001
Calcaneus BMD (g/cm2)	0.509 (0.4675–0.532)	0.375 (0.303–0.411)	0.393 (0.344–0.44)	0.374 (0.303–0.379)	0.003

The trabecular bone score values were decreased by 17% in the patients compared with the controls. Patients with and without a history of low-trauma vertebral fractures had similar trabecular bone score values (p = 0.75).

The liver enzymes were increased in the patient group, and the coagulation parameters were altered in terms of prolonged partial thromboplastin time with increased international normalized ratio. The patients had higher serum phosphorus and lower adjusted calcium values, even though both parameters were within normal ranges. 25-OH vitamin D and CTX were similar, and both P1NP and alkaline phosphatase were higher in the patient group (Table 4).

Table 4

Clinical chemistry and serum markers of bone turnover. There were no differences between alcoholic and non-alcoholic cirrhosis except higher alanine aminotransferase in non-alcoholic cirrhosis (p = 0.043). iPTH, intact parathyroid hormone; CTX, C-terminal telopeptide of type I collagen; P1NP, procollagen type 1 N-terminal peptide

	Controls median values (Q1–Q3)	Cirrhosis median values (Q1–Q3)	Alcoholic liver disease median values (Q1–Q3)	Non-alcoholic median values (Q1–Q3)	p value cirrhosis vs. controls	Normal range
Adjusted total calcium (mmol/l)	2.34 (2.27–2.41)	2.27 (2.20–2.38)	2.26 (2.21–2.38)	2.29 (2.19–2.36)	0.045	2.1–2.58
Phosphate (mmol/l)	1 (0.9–1.11)	1.16 (1.005–1.268)	1.17 (1.01–1.3)	1.16 (1–1.26)	0.003	0.6–1.55
iPTH (pg/ml)	48 (34.6–54.3)	50.4 (38.5–63.4)	58.2 (41.4–63.4)	48.6 (33.5–62.8)	0.265	15–65
25-OH vitamin D (ng/ml)	22.47 (16.3–30.4)	20.9 (13.6–27)	23.6 (14.3–29.8)	18.12 (13.11–24.73)	0.434	> 20
CTX (ng/ml)	0.297 (0.219–0.361)	0.255 (0.173–0.567)	0.231 (0.163–0.573)	0.259 (0.221–0.553)	0.142	0.14–0.68
P1NP (μg/l)	41.2 (39.3–45.9)	57.5 (38.9–78.9)	39.1 (32.2–67.6)	70 (59.1–78.8)	0.010	16–67
Alkaline phosphatase (U/l)	72 (62.5–85.5)	111 (79.3–171)	110 (75.8–171)	108 (83–186)	< 0.001	< 104
Albumin (g/dl)	4.37 (4.15–4.52)	3.84 (3.46–4.29)	4.12 (3.75–4.41)	3.55 (3.32–4.04)	0.001	3.5–5.0
Bilirubin (mg/dl)	0.5 (0.44–0.75)	1.05 (0.6–1.75)	0.8 (0.58–1.23)	1.35 (0.98–2.63)	0.001	< 1.2
Aspartate transaminase (U/l)	22.5 (19–26.3)	42 (29.8–58.3)	34 (26–45.3)	49 (36.3–72.8)	< 0.001	< 35
Alanine aminotransferase (U/l)	22 (16–34)	30.5 (17.5–49.5)	23 (15.8–32.8)	45.5 (23.3–56.3)	0.042	< 35
Gamma-glutamyl transferase (U/l)	27 (16–37)	153 (57–295)	188 (72–297)	110 (57–229)	< 0.001	< 40
Partial thromboplastin time (s)	30 (25.6–32.4)	33.7 (31.8–36.5)	32.85 (32–35.9)	34.7 (31–37.2)	< 0.001	25–35
International normalized ratio	0.9 (0.9–1)	1.1 (1.075–1.3)	1.1 (0.98–1.2)	1.1 (1.1–1.3)	< 0.001	< 1.1
Creatinine (mg/dl)	0.85 (0.76–1)	0.8 (0.7–0.968)	0.8 (0.76–1.02)	0.75 (0.69–0.91)	0.710	0.6–1.2

No differences in bone microarchitecture, markers of bone turnover, aBMD, or the trabecular bone score were observed between subjects with alcoholic liver disease and the non-alcoholic group.

Correlations (regression analysis)

As calculated by multiple regression analysis, significant correlations between most parameters of trabecular and cortical microarchitecture (except cortical BMD) were observed with aBMD of the lumbar spine and hip. The strongest correlation was observed for tibial Tb.BV/TV (R2 = 0.5, p < 0.001) and tibial Tb.N (R2 = 0.51, p < 0.001).

In the multiple regression analysis, there was no significant correlation between microarchitecture and the Child-Pugh score or the MELD score, even when adjusted for both aBMD (lumbar spine, hip, total body, and radius) and serum markers of bone turnover (alkaline phosphatase, iPTH, and CTX). Therefore, differences in microarchitecture were independent of disease severity (e.g., tibial Tb.BV/TV F2 = 0.09, p = 0.77; tibial Tb.N F2 = 0.79, p = 0.38).

Discussion

To date, this is the first study to evaluate bone microarchitecture in hepatic cirrhosis via state-of-the-art non-invasive HR-pQCT. We observed significantly impaired microarchitecture at both the trabecular and cortical compartments, as well as differences in aBMD and serum markers of bone turnover.

To our knowledge, only two studies have performed histomorphometry in patients with cirrhosis [6, 7]. Other investigations in patients with chronic liver disease varied concerning etiology and severity [15, 16]. Additionally, no detailed parameters of cortical microstructure or bone geometry have so far been evaluated in patients with cirrhosis. Dynamic histomorphometry in cirrhosis has suggested reduced formation, increased resorption, and normal mineralization [6] with no signs of osteomalacia [7].

We assessed bone microarchitecture at both weight-bearing and non-weight-bearing sites and observed increased Ct.Po and decreased Ct.Th in hepatic cirrhosis. Ct.Th and Ct.Po are closely related to cortical integrity and bone strength [17]. Thus, Ct.Th is decreased in chronic kidney disease patients with sustained fractures [18] and Ct.Po increased in diabetics, especially in those with prevalent fragility fractures [19]. Moreover, we observed impaired trabecular microarchitecture with decreased Tb.BV/TV and a trend towards lower Tb.Th and elevated Tb.Sp. Impaired trabecular microarchitecture is associated with fragility fractures; chronic kidney disease patients who had sustained fractures have lower Tb.BV/TV, and Tb.N is decreased with increased Tb.Sp [18].

Based on the deteriorations in both trabecular and cortical microarchitecture, we assume increased susceptibility for fragility fractures in hepatic cirrhosis. Recent studies have identified both vertebral and non-vertebral fractures in a high percentage of patients with chronic liver disease, the prevalence varying between 7 and 35% [20]. Moreover, in up to 49% of the patients with advanced liver disease, there was at least one self-reported low-trauma fracture [21], and in a study on hypogonadal cirrhotic patients, 71% had sustained vertebral or non-vertebral fractures, including the hip, regardless of trauma intensity [22]. Compared with these studies, a relatively high percentage of our patients (40%) had sustained at least one low-trauma fracture. However, not all of the previous studies have incorporated spinal X-rays, and vertebral fractures may have, thus, been underdiagnosed.

In line with current studies investigating patients in the same age group [2, 3], we observed lower vertebral aBMD. In contrast to other investigations [2-4], we also found decreased aBMD at the total hip, femoral neck, the total body scan, as well as at the calcaneus, implying that hepatic cirrhosis affects the entire skeleton, independent of etiology.

Trabecular bone score, which is automatically calculated from gray scale levels derived from DXA measurements, is capable of reflecting vertebral trabecular microarchitecture [23]. Lower trabecular bone scores accurately predict impaired trabecular microarchitecture in various patient groups, such as those with pre- or postmenopausal osteoporosis [23], osteogenesis imperfecta [24], chronic kidney disease [25], or diabetes [26]. To date, this is the first evaluation of trabecular bone score in hepatic cirrhosis and, compared with the aforementioned patient groups with increased fracture risk, trabecular bone score was even more strongly decreased in our patients (17%).

In hepatic cirrhosis, several metabolic alterations contribute to bone loss, including increased oncofetal fibronectin [27]; decreased levels of insulin-like growth factor 1 synthesized in the liver [28]; upregulation of proinflammatory cytokines [28]; chronic cholestasis with retained bilirubin and bile acids [29]; hypogonadism [30]; and higher alcohol consumption, smoking, lower physical activity, and malnutrition with muscle wasting [28].

Low total calcium levels in hepatic cirrhosis are common in the literature [6, 31], even though other authors have reported normal levels [2]. In our patients, the adjusted total calcium levels were within normal ranges but significantly lower than in the controls. In addition, phosphorus levels were higher in our patients than the controls but still within normal ranges. In a normal population, higher phosphorus levels within normal ranges were related to increased fracture risk [32]. In our study, iPTH, 25-OH vitamin D, and CTX were similar in the patients and the controls and thereby unlikely to explain increased phosphorus levels. Finally, patients with hepatic cirrhosis are likely to consume high quantities of processed food containing high amounts of phosphorus additives [33].

In our patients, the standard serum markers of bone turnover (CTX, P1NP) did not reflect the unfavorable changes that lead to the deterioration of both cortical and trabecular microarchitecture. Increased P1NP levels in our patients could be explained by altered hepatic collagen metabolism [34]. Therefore, collagen-based serum parameters do not appear to be valuable markers of bone turnover in hepatic cirrhosis.

As tested by multiple regression analysis, we observed no correlation between bone microarchitecture and the severity of liver disease, as reflected by the Child-Pugh or MELD scores, even when adjusted for aBMD and serum markers of bone turnover. These findings indicate a loss of structural integrity already at the early stages. Similarly, histomorphometric studies have reported no differences between different Child-Pugh stages [7].

The impact of the etiology of cirrhosis is under debate in the literature. Some studies have observed no differences in fracture risk [1], but others have suggested stronger bone loss in cholestatic than viral cirrhosis [35].

Similar to our work, a recent study in non-cirrhotic primary biliary cholangitis patients of a similar age [36] observed deranged microarchitecture of both cortical and trabecular bones. In detail, Ct.Th, BV/TV, and Tb.Th were decreased with unchanged Tb.N at both the radius and tibia. Ct.Po and serum markers of bone turnover were not determined. In contrast to our findings, disease stage was related to the degree of microstructural derangement.

Some limitations of this study are to be mentioned. First, we were unable to precisely define the duration of hepatic cirrhosis. However, we provided Child-Pugh and MELD scores to reflect disease severity. Second, we were dependent on the subjects’ statements concerning units of alcohol intake and packyears of smoking. Third, due to technical issues, we could not determine trabecular bone score and calcaneal aBMD in all subjects. Fourth, we determined only one parameter of bone formation (P1NP). Fifth, the threshold-based algorithm of image segmentation could possibly have had an influence on the portions of trabecular and cortical bone. Sixth, our study possibly lacked the power to test for differences between different etiologies. Finally, we could have underestimated differences in Ct.Po, as smaller pores are possibly not recognized at a voxel size of 82 μm.

In conclusion, both cortical and cancellous bone microarchitectures were affected at all assessed sites and structural deteriorations were independent of disease severity. Especially at the tibia, cortical bone was affected with both decreased Ct.Th and increased Ct.Po. Areal BMD was diminished at all measured sites as a sign of generalized bone disease in patients with hepatic cirrhosis. This is supported by differences in bone microarchitecture, non-invasively measured by HR-pQCT. A clear statement of this study is the implementation of DXA as an easily obtainable screening method for osteoporosis in this patient group to evaluate an individual fracture risk. We, therefore, generally recommend osteologic screening, including DXA of the lumbar spine and hip, for patients with hepatic cirrhosis, independent of its etiology or disease severity.