Literature DB >> 30786231

Membrane Electroporation and Electropermeabilization: Mechanisms and Models.

Tadej Kotnik1, Lea Rems2, Mounir Tarek3, Damijan Miklavčič1.   

Abstract

Exposure of biological cells to high-voltage, short-duration electric pulses causes a transient increase in their plasma membrane permeability, allowing transmembrane transport of otherwise impermeant molecules. In recent years, large steps were made in the understanding of underlying events. Formation of aqueous pores in the lipid bilayer is now a widely recognized mechanism, but evidence is growing that changes to individual membrane lipids and proteins also contribute, substantiating the need for terminological distinction between electroporation and electropermeabilization. We first revisit experimental evidence for electrically induced membrane permeability, its correlation with transmembrane voltage, and continuum models of electropermeabilization that disregard the molecular-level structure and events. We then present insights from molecular-level modeling, particularly atomistic simulations that enhance understanding of pore formation, and evidence of chemical modifications of membrane lipids and functional modulation of membrane proteins affecting membrane permeability. Finally, we discuss the remaining challenges to our full understanding of electroporation and electropermeabilization.

Entities:  

Keywords:  aqueous pores in lipid bilayers; cell membrane; molecular dynamics; pulsed electric field treatment; theoretical model; transmembrane molecular transport

Mesh:

Substances:

Year:  2019        PMID: 30786231     DOI: 10.1146/annurev-biophys-052118-115451

Source DB:  PubMed          Journal:  Annu Rev Biophys        ISSN: 1936-122X            Impact factor:   12.981


  70 in total

1.  An experimental system for real-time fluorescence recordings of cell membrane changes induced by electroporation.

Authors:  Ioan Tivig; Tudor Savopol; Eugenia Kovacs; Mihaela G Moisescu
Journal:  Eur Biophys J       Date:  2019-12-23       Impact factor: 1.733

2.  Gene delivery in adherent and suspension cells using the combined physical methods.

Authors:  Kimia Kardani; Alireza Milani; Azam Bolhassani
Journal:  Cytotechnology       Date:  2022-02-03       Impact factor: 2.058

3.  Transcriptional effects of electroporation on Echinococcus multilocularis primary cell culture.

Authors:  Matías Gastón Pérez; Natalia Rego; Markus Spiliotis; Klaus Brehm; Mara Cecilia Rosenzvit
Journal:  Parasitol Res       Date:  2022-01-26       Impact factor: 2.289

4.  Identification of electroporation sites in the complex lipid organization of the plasma membrane.

Authors:  Xinru Tang; Fangwei Zhao; Lea Rems; Sergio Pérez-Conesa; Ilaria Testa; Lucie Delemotte
Journal:  Elife       Date:  2022-02-23       Impact factor: 8.140

Review 5.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

Review 6.  [Pulmonary vein isolation in atrial fibrillation using pulsed field ablation].

Authors:  F Lindemann; S Nedios; T Seewöster; G Hindricks
Journal:  Herz       Date:  2021-06-17       Impact factor: 1.443

7.  Determining tissue conductivity in tissue ablation by nanosecond pulsed electric fields.

Authors:  Edwin A Oshin; Siqi Guo; Chunqi Jiang
Journal:  Bioelectrochemistry       Date:  2021-09-20       Impact factor: 5.373

8.  Generation of Tumor-activated T cells Using Electroporation.

Authors:  Nastaran Alinezhadbalalami; Philip M Graybill; Khan Mohammad Imran; Scott S Verbridge; Irving C Allen; Rafael V Davalos
Journal:  Bioelectrochemistry       Date:  2021-07-13       Impact factor: 5.373

9.  10 ns PEFs induce a histological response linked to cell death and cytotoxic T-lymphocytes in an immunocompetent mouse model of peritoneal metastasis.

Authors:  A Taibi; M-L Perrin; J Albouys; J Jacques; C Yardin; S Durand-Fontanier; S M Bardet
Journal:  Clin Transl Oncol       Date:  2021-03-07       Impact factor: 3.405

Review 10.  Permeabilizing Cell Membranes with Electric Fields.

Authors:  Alondra A Aguilar; Michelle C Ho; Edwin Chang; Kristen W Carlson; Arutselvan Natarajan; Tal Marciano; Ze'ev Bomzon; Chirag B Patel
Journal:  Cancers (Basel)       Date:  2021-05-10       Impact factor: 6.639

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