Literature DB >> 30783970

In Situ Hybridization for Detection of AAV-Mediated Gene Expression.

Jacqueline E Hunter1, Brittney L Gurda1, Sea Young Yoon1,2, Michael J Castle3, John H Wolfe4,5,6.   

Abstract

Techniques to localize vector transgenes in cells and tissues are essential in order to fully characterize gene therapy outcomes. In situ hybridization (ISH) uses synthesized complementary RNA or DNA nucleotide probes to localize and detect sequences of interest in fixed cells, tissue sections, or whole tissue mounts. Variations in techniques include adding labels to probes, such as fluorophores, which can allow for the simultaneous visualization of multiple targets. Here we provide the steps necessary to: (1) label probes for colorimetric visualization and (2) perform ISH on OCT cryo-preserved fixed frozen tissues.

Entities:  

Keywords:  Cell-specific labeling; DIG labeling; FISH; Fluorescence in situ hybridization; Hybridization; ISH; In situ hybridization; Riboprobes

Mesh：

Substances：

Year:  2019        PMID： 30783970      PMCID： PMC7029785          DOI： 10.1007/978-1-4939-9139-6_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  24 in total

1.  Widespread gene delivery and structure-specific patterns of expression in the brain after intraventricular injections of neonatal mice with an adeno-associated virus vector.

Authors:  M A Passini; J H Wolfe
Journal:  J Virol       Date:  2001-12       Impact factor: 5.103

Review 2.  Fluorescence in situ hybridization: past, present and future.

Authors:  Jeffrey M Levsky; Robert H Singer
Journal:  J Cell Sci       Date:  2003-07-15       Impact factor: 5.285

3.  Distribution of a lysosomal enzyme in the adult brain by axonal transport and by cells of the rostral migratory stream.

Authors:  Marco A Passini; Edward B Lee; Gregory G Heuer; John H Wolfe
Journal:  J Neurosci       Date:  2002-08-01       Impact factor: 6.167

4.  Transduction characteristics of adeno-associated virus vectors expressing cap serotypes 7, 8, 9, and Rh10 in the mouse brain.

Authors:  Cassia N Cearley; John H Wolfe
Journal:  Mol Ther       Date:  2006-01-18       Impact factor: 11.454

5.  Clinical Improvement of Alpha-mannosidosis Cat Following a Single Cisterna Magna Infusion of AAV1.

Authors:  Sea Young Yoon; Jessica H Bagel; Patricia A O'Donnell; Charles H Vite; John H Wolfe
Journal:  Mol Ther       Date:  2015-09-10       Impact factor: 11.454

6.  Long-term AAV vector gene and protein expression in mouse brain from a small pan-cellular promoter is similar to neural cell promoters.

Authors:  T Husain; M A Passini; M K Parente; N W Fraser; J H Wolfe
Journal:  Gene Ther       Date:  2009-05-21       Impact factor: 5.250

7.  A single injection of an adeno-associated virus vector into nuclei with divergent connections results in widespread vector distribution in the brain and global correction of a neurogenetic disease.

Authors:  Cassia N Cearley; John H Wolfe
Journal:  J Neurosci       Date:  2007-09-12       Impact factor: 6.167

8.  Widespread correction of lysosomal storage in the mucopolysaccharidosis type VII mouse brain with a herpes simplex virus type 1 vector expressing beta-glucuronidase.

Authors:  Bradford K Berges; Srikanth Yellayi; Brian A Karolewski; Richard R Miselis; John H Wolfe; Nigel W Fraser
Journal:  Mol Ther       Date:  2006-03-03       Impact factor: 11.454

9.  Expanded repertoire of AAV vector serotypes mediate unique patterns of transduction in mouse brain.

Authors:  Cassia N Cearley; Luk H Vandenberghe; Michael K Parente; Erin R Carnish; James M Wilson; John H Wolfe
Journal:  Mol Ther       Date:  2008-08-19       Impact factor: 11.454

10.  Adeno-associated virus serotypes 1, 8, and 9 share conserved mechanisms for anterograde and retrograde axonal transport.

Authors:  Michael J Castle; Zachary T Gershenson; April R Giles; Erika L F Holzbaur; John H Wolfe
Journal:  Hum Gene Ther       Date:  2014-05-02       Impact factor: 5.695
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  2 in total

1.  Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing.

Authors:  David Brown; Michael Altermatt; Tatyana Dobreva; Sisi Chen; Alexander Wang; Matt Thomson; Viviana Gradinaru
Journal:  Front Immunol       Date:  2021-10-21       Impact factor: 7.561

2.  High-Resolution Histological Landscape of AAV DNA Distribution in Cellular Compartments and Tissues following Local and Systemic Injection.

Authors:  Junling Zhao; Yongping Yue; Aman Patel; Lakmini Wasala; Jacob F Karp; Keqing Zhang; Dongsheng Duan; Yi Lai
Journal:  Mol Ther Methods Clin Dev       Date:  2020-08-11       Impact factor: 6.698
  2 in total

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