HS- facilitated sulfur pyran realizing hydrogen sulfide detection and imaging in HepG2 cells and chlorella.

The new carbazole-based fluorescent probe CA-1 was designed and synthesized for the high selective detection of H2S based on HS- facilitated sulfur pyran resulting in UV-Vis and fluorescent spectra changes. At the same time, the probe showed good sensitivity to the detection of H2S with a low detection limit of 0.16 μM. The detection process can be monitored by naked eye: with the addition of H2S, the probe system changed from yellow to pink. Importantly, the probe could be applied in monitoring H2S in HepG2 cells and Chlorella. These results indicate that CA-1 can be used as a promising fluorescent probe for the detection of H2S in situ.