Multi-templates based molecularly imprinted sodium alginate/MnO2 for simultaneous enantiorecognition of lysine, alanine and cysteine isomers.

Sodium alginate (SA) was electrodeposited onto the surface of a glassy carbon electrode (GCE), and then l-lysine (l-Lys), l-alanine (l-Ala) and l-cysteine (l-Cys) were simultaneously introduced to the surface of SA via electrostatic attractions. γ-MnO2 film was deposited potentiostatically onto the multi-templates immobilized SA from an aqueous mixture of K2SO4 and MnSO4. The templates of l-Lys, l-Ala and l-Cys were then oxidized and removed by cyclic voltammetry (CV) while maintaining the crystalline structures of SA and MnO2. Finally, the molecularly imprinted SA/MnO2 was successfully applied in the simultaneous recognition of the isomers of Lys, Ala and Cys by differential pulse voltammetry (DPV). This work opens a new avenue in the construction of SA-based multi-templates molecularly imprinted systems for electrochemical simultaneous recognition of the isomers of several chiral amino acids.