Literature DB >> 30775737

A novel template repairing-PCR (TR-PCR) reaction platform for microRNA detection using translesional synthesis on DNA templates containing abasic sites.

Dianming Zhou1, Xiaohui Lin, Weichen Gao, Jiafang Piao, Shufei Li, Ning He, Zhiyong Qian, Miao Zhao, Xiaoqun Gong.   

Abstract

A novel template repairing-PCR technology based on miRNA-primed bypass synthesis at the abasic sites on the PCR template is developed as a sensitive and selective platform for miRNA detection. The assay is expected to show great promise for reverse transcription-free RNA PCR amplification and target-based coding analysis.

Mesh:

Substances:

Year:  2019        PMID: 30775737     DOI: 10.1039/c8cc10226k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  1 in total

1.  Signal-On Fluorescence Biosensor for Highly Sensitive Detection of miRNA-21 Based on DNAzyme Assisted Double-Hairpin Molecular Beacon.

Authors:  Chenxin Fang; Yuxing Yang; Shuhao Zou; Ping Ouyang; Yang Qing; Jialun Han; Haiyu Li; Zhencui Wang; Jie Du
Journal:  Biosensors (Basel)       Date:  2022-04-27
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.