Damla Binnetoglu1, Ahmet Hacimuftuoglu2, Feyza Aricioglu3. 1. Department of Medical Pharmacology, Faculty of Medicine, Kafkas University, Kars, Turkey. Electronic address: damlacetin.erz@gmail.com. 2. Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. 3. Department of Pharmacology and Psychopharmacology Research Unit, Marmara University School of Pharmacy, Istanbul, Turkey.
Abstract
AIMS: The effects of agmatine, an endogenous substance known to have a neuroprotective effect against neurotoxicity has been investigated. MATERIAL AND METHODS: The primary neuron culture obtained from neonatal rats was exposed to toxicity with paclitaxel and cisplatin and the effect of agmatine on both acute (1 h) and chronic (24 h) exposure was demonstrated by biochemical and molecular analyses. It was demonstrated that the effect of agmatine before and after agmatine was induced by neurotoxicity before agmatine and the effect of agmatine on the formed and occuring toxicities. In addition to the results of cell viability assay, total oxidant capacity and total antioxidant capacity, we have found the opportunity to elaborate on our molecular mechanisms by elaborating our findings with apoptotic and inflammation markers such as caspase 3, kaspase 9 and TNF alpha. KEY FINDINGS: The results of our study revealed the effect profile of a protective molecule against pathological neural deaths due to neurodegeneration not only in neurotoxicity due to anticancer drugs. SIGNIFICANCE: In this context, we tried to reverse neurotoxicity due to anticancer drugs by using agmatine the duration (1 and 24 h) and dosage (10-5 M and 10-6 M) determined.
AIMS: The effects of agmatine, an endogenous substance known to have a neuroprotective effect against neurotoxicity has been investigated. MATERIAL AND METHODS: The primary neuron culture obtained from neonatal rats was exposed to toxicity with paclitaxel and cisplatin and the effect of agmatine on both acute (1 h) and chronic (24 h) exposure was demonstrated by biochemical and molecular analyses. It was demonstrated that the effect of agmatine before and after agmatine was induced by neurotoxicity before agmatine and the effect of agmatine on the formed and occuring toxicities. In addition to the results of cell viability assay, total oxidant capacity and total antioxidant capacity, we have found the opportunity to elaborate on our molecular mechanisms by elaborating our findings with apoptotic and inflammation markers such as caspase 3, kaspase 9 and TNF alpha. KEY FINDINGS: The results of our study revealed the effect profile of a protective molecule against pathological neural deaths due to neurodegeneration not only in neurotoxicity due to anticancer drugs. SIGNIFICANCE: In this context, we tried to reverse neurotoxicity due to anticancer drugs by using agmatine the duration (1 and 24 h) and dosage (10-5 M and 10-6 M) determined.
Authors: Khalid S Hashem; Asmaa Mohammed M Hussein Elkelawy; Saber Abd-Allah; Nermeen A Helmy Journal: Iran J Basic Med Sci Date: 2020-04 Impact factor: 2.699