Literature DB >> 30770403

The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE.

Ying Li Liu1,2, Tian Tian He1,2, Lu Yi Liu1, Jia Yi1,2, Pin Nie1,3,4, Hong Bing Yu5, Hai Xia Xie6.   

Abstract

The type III secretion system (T3SS) is one of the most important virulence factors of the fish pathogen Edwardsiella piscicida It contains three translocon proteins, EseB, EseC, and EseD, required for translocation of effector proteins into host cells. We have previously shown that EseB forms filamentous appendages on the surface of E. piscicida, and these filamentous structures mediate bacterial cell-cell interactions promoting autoaggregation and biofilm formation. In the present study, we show that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida At 18 h postsubculture, a ΔeseC strain developed strong autoaggregation and mature biofilm formation, accompanied by enhanced formation of EseB filamentous appendages. This is in contrast to the weak autoaggregation and immature biofilm formation seen in the E. piscicida wild-type strain. EseE, a protein that directly binds to EseC and also positively regulates the transcription of the escC-eseE operon, was liberated and showed increased levels in the absence of EseC. This led to augmented transcription of the escC-eseE operon, thereby increasing the steady-state protein levels of intracellular EseB, EseD, and EseE, as well as biofilm formation. Notably, the levels of intracellular EseB and EseD produced by the ΔeseE and ΔeseC ΔeseE strains were similar but remarkably lower than those produced by the wild-type strain at 18 h postsubculture. Taken together, we have shown that the translocon protein EseC inhibits biofilm formation through sequestering EseE, a positive regulator of the escC-eseE operon.IMPORTANCE Edwardsiella piscicida, previously known as Edwardsiella tarda, is a Gram-negative intracellular pathogen that mainly infects fish. The type III secretion system (T3SS) plays a pivotal role in its pathogenesis. The T3SS translocon protein EseB is required for the assembly of filamentous appendages on the surface of E. piscicida The interactions between the appendages facilitate autoaggregation and biofilm formation. In this study, we explored the role of the other two translocon proteins, EseC and EseD, in biofilm formation. We have demonstrated that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida, providing new insights into the regulatory mechanism involved in E. piscicida biofilm formation.
Copyright © 2019 American Society for Microbiology.

Entities:  

Keywords:  Edwardsiella piscicida; biofilm; translocon protein; type III secretion system

Mesh:

Substances:

Year:  2019        PMID: 30770403      PMCID: PMC6450016          DOI: 10.1128/AEM.02133-18

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  32 in total

1.  Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression.

Authors:  R A Edwards; L H Keller; D M Schifferli
Journal:  Gene       Date:  1998-01-30       Impact factor: 3.688

2.  Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction.

Authors:  R H Valdivia; S Falkow
Journal:  Mol Microbiol       Date:  1996-10       Impact factor: 3.501

3.  Edwardsiella tarda EscE (Orf13 Protein) Is a Type III Secretion System-Secreted Protein That Is Required for the Injection of Effectors, Secretion of Translocators, and Pathogenesis in Fish.

Authors:  Jin Fang Lu; Wei Na Wang; Gai Ling Wang; He Zhang; Ying Zhou; Zhi Peng Gao; Pin Nie; Hai Xia Xie
Journal:  Infect Immun       Date:  2015-10-12       Impact factor: 3.441

4.  Edwardsiella tarda EsaE (Orf19 protein) is required for the secretion of type III substrates, and pathogenesis in fish.

Authors:  Ying Zhou; Lu Yi Liu; Tian Tian He; Zubair Ahmed Laghari; Pin Nie; Qian Gao; Hai Xia Xie
Journal:  Vet Microbiol       Date:  2016-05-09       Impact factor: 3.293

5.  EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD.

Authors:  Jun Zheng; Nan Li; Yuen Peng Tan; J Sivaraman; Yu-Keung Mok; Zhao Lan Mo; Ka Yin Leung
Journal:  Microbiology (Reading)       Date:  2007-06       Impact factor: 2.777

6.  Biofilms and type III secretion are not mutually exclusive in Pseudomonas aeruginosa.

Authors:  H Mikkelsen; N J Bond; M E Skindersoe; M Givskov; K S Lilley; M Welch
Journal:  Microbiology       Date:  2009-03       Impact factor: 2.777

7.  Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence.

Authors:  Huifang Cao; Cuiting Yang; Shu Quan; Tianjian Hu; Lingzhi Zhang; Yuanxing Zhang; Dahai Yang; Qin Liu
Journal:  Cell Microbiol       Date:  2017-11-02       Impact factor: 3.715

8.  Multi-Functional Characteristics of the Pseudomonas aeruginosa Type III Needle-Tip Protein, PcrV; Comparison to Orthologs in other Gram-negative Bacteria.

Authors:  Hiromi Sato; Dara W Frank
Journal:  Front Microbiol       Date:  2011-07-04       Impact factor: 5.640

9.  The SseC translocon component in Salmonella enterica serovar Typhimurium is chaperoned by SscA.

Authors:  Colin A Cooper; David T Mulder; Sarah E Allison; Ana Victoria C Pilar; Brian K Coombes
Journal:  BMC Microbiol       Date:  2013-10-04       Impact factor: 3.605

10.  The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation.

Authors:  Tamara Zimaro; Ludivine Thomas; Claudius Marondedze; Germán G Sgro; Cecilia G Garofalo; Florencia A Ficarra; Chris Gehring; Jorgelina Ottado; Natalia Gottig
Journal:  BMC Microbiol       Date:  2014-04-18       Impact factor: 3.605

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  2 in total

1.  HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida.

Authors:  Yan-Jie Shi; Qing-Jian Fang; Hui-Qin Huang; Chun-Guang Gong; Yong-Hua Hu
Journal:  Vet Res       Date:  2019-10-02       Impact factor: 3.683

Review 2.  Bacteria autoaggregation: how and why bacteria stick together.

Authors:  El-Shama Q A Nwoko; Iruka N Okeke
Journal:  Biochem Soc Trans       Date:  2021-06-30       Impact factor: 5.407

  2 in total

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