Zhihuan Tian1, Chuanzhen Jiao2. 1. College of Yingdong Life Science, Shaoguan University, Room C-304, Yingdong Building, No. 288, Daxue Road, Zhenjiang District, Shaoguan, 512005, Guangdong, China. 2. College of Yingdong Life Science, Shaoguan University, Room C-304, Yingdong Building, No. 288, Daxue Road, Zhenjiang District, Shaoguan, 512005, Guangdong, China. jiaocz@gmail.com.
Abstract
BACKGROUND: Molting is a critical developmental process for crustaceans, during which the claw muscles undergo periodic atrophy and restoration. But the mechanism underlying this special muscle reshuffle around ecdysis is not yet thoroughly understood. OBJECTIVE: To investigate the molecular mechanism underlying the muscle's reshuffle over the molting cycle of Chinese mitten crab Eriocheir sinensis. METHODS: The Illumina high-throughput sequencing technique were used to sequence the transcriptome of the whole claw muscles from Chinese mitten crab Eriocheir sinensis in three molting stages (inter-molt C stage, pre-molt D3-4 and post-molt A-B stage); the de novo assembly, annotation and functional evaluation of the contigs were performed with bioinformatics tools. RESULTS: Totally 129,149 unigenes, 128,190 CDS, 33,770 SSRs and a large number of SNP sites were obtained, and 3700 and 12,771 differentially expressed genes (DEGs) were identified respectively in A-B and D3-4 stage compared with that in C stage. The identified DEGs were enriched to 746 and 1 408 GO terms respectively in A-B and D3-4 stage compared with C stage (p ≤ 0.05). KEGG pathway analysis showed that the DEGs were significantly enriched in 14 and 11 pathways in A-B vs C comparison and D3-4 vs C comparison (p ≤ 0.05), respectively. These DEGs are involved in several biological processes critical for the animal's growth and development, such as gene expression, protein synthesis, muscle development, new cuticle reconstruction, oxidation-reduction process and glycolytic process. CONCLUSION: The data generated in this study is the first transcriptomic resource from the muscles of Chinese mitten crab, which would facilitate to characterize key molecular processes underlying crab muscle's growth and development during the molting cycles.
BACKGROUND: Molting is a critical developmental process for crustaceans, during which the claw muscles undergo periodic atrophy and restoration. But the mechanism underlying this special muscle reshuffle around ecdysis is not yet thoroughly understood. OBJECTIVE: To investigate the molecular mechanism underlying the muscle's reshuffle over the molting cycle of Chinese mitten crabEriocheir sinensis. METHODS: The Illumina high-throughput sequencing technique were used to sequence the transcriptome of the whole claw muscles from Chinese mitten crabEriocheir sinensis in three molting stages (inter-molt C stage, pre-molt D3-4 and post-molt A-B stage); the de novo assembly, annotation and functional evaluation of the contigs were performed with bioinformatics tools. RESULTS: Totally 129,149 unigenes, 128,190 CDS, 33,770 SSRs and a large number of SNP sites were obtained, and 3700 and 12,771 differentially expressed genes (DEGs) were identified respectively in A-B and D3-4 stage compared with that in C stage. The identified DEGs were enriched to 746 and 1 408 GO terms respectively in A-B and D3-4 stage compared with C stage (p ≤ 0.05). KEGG pathway analysis showed that the DEGs were significantly enriched in 14 and 11 pathways in A-B vs C comparison and D3-4 vs C comparison (p ≤ 0.05), respectively. These DEGs are involved in several biological processes critical for the animal's growth and development, such as gene expression, protein synthesis, muscle development, new cuticle reconstruction, oxidation-reduction process and glycolytic process. CONCLUSION: The data generated in this study is the first transcriptomic resource from the muscles of Chinese mitten crab, which would facilitate to characterize key molecular processes underlying crab muscle's growth and development during the molting cycles.
Authors: John K Colbourne; Michael E Pfrender; Donald Gilbert; W Kelley Thomas; Abraham Tucker; Todd H Oakley; Shinichi Tokishita; Andrea Aerts; Georg J Arnold; Malay Kumar Basu; Darren J Bauer; Carla E Cáceres; Liran Carmel; Claudio Casola; Jeong-Hyeon Choi; John C Detter; Qunfeng Dong; Serge Dusheyko; Brian D Eads; Thomas Fröhlich; Kerry A Geiler-Samerotte; Daniel Gerlach; Phil Hatcher; Sanjuro Jogdeo; Jeroen Krijgsveld; Evgenia V Kriventseva; Dietmar Kültz; Christian Laforsch; Erika Lindquist; Jacqueline Lopez; J Robert Manak; Jean Muller; Jasmyn Pangilinan; Rupali P Patwardhan; Samuel Pitluck; Ellen J Pritham; Andreas Rechtsteiner; Mina Rho; Igor B Rogozin; Onur Sakarya; Asaf Salamov; Sarah Schaack; Harris Shapiro; Yasuhiro Shiga; Courtney Skalitzky; Zachary Smith; Alexander Souvorov; Way Sung; Zuojian Tang; Dai Tsuchiya; Hank Tu; Harmjan Vos; Mei Wang; Yuri I Wolf; Hideo Yamagata; Takuji Yamada; Yuzhen Ye; Joseph R Shaw; Justen Andrews; Teresa J Crease; Haixu Tang; Susan M Lucas; Hugh M Robertson; Peer Bork; Eugene V Koonin; Evgeny M Zdobnov; Igor V Grigoriev; Michael Lynch; Jeffrey L Boore Journal: Science Date: 2011-02-04 Impact factor: 47.728