AIM: Reconstruction of bone defects with autologous grafts has certain disadvantages. The aim of this study is to introduce a new type of living bioreactor for engineering of bone flaps and to evaluate the effect of different barrier membranes. MATERIALS & METHODS: Scaffolds loaded with bone morphogenetic proteins and bone marrow aspirate wrapped with either a collagen membrane or a periosteal flap were implanted in the greater omentum of miniature pigs. RESULTS: Both histological and radiographic evaluation showed proven bone formation and increased density after 8 and 16 weeks, with an enhanced effect of the periosteal transplant. CONCLUSION: The greater omentum is a suitable bioreactor for bone tissue engineering. Endocultivation is both an innovative and promising approach in regenerative medicine.
AIM: Reconstruction of bone defects with autologous grafts has certain disadvantages. The aim of this study is to introduce a new type of living bioreactor for engineering of bone flaps and to evaluate the effect of different barrier membranes. MATERIALS & METHODS: Scaffolds loaded with bone morphogenetic proteins and bone marrow aspirate wrapped with either a collagen membrane or a periosteal flap were implanted in the greater omentum of miniature pigs. RESULTS: Both histological and radiographic evaluation showed proven bone formation and increased density after 8 and 16 weeks, with an enhanced effect of the periosteal transplant. CONCLUSION: The greater omentum is a suitable bioreactor for bone tissue engineering. Endocultivation is both an innovative and promising approach in regenerative medicine.
Entities:
Keywords:
bone morphogenetic protein; bone tissue engineering; endocultivation; living bioreactor; periosteum; scaffolds
Authors: A G Abu-Shahba; T Wilkman; R Kornilov; M Adam; K M Salla; J Lindén; A K Lappalainen; R Björkstrand; R Seppänen-Kaijansinkko; B Mannerström Journal: J Dent Res Date: 2021-09-11 Impact factor: 6.116