Liang Cheng1, Fan Yang2, Xin Cao3, Guo-Qing Li3, Ting-Ting Lu3, Yun-Qing Zhu3, Yun Hu4, Xiao-Ming Mao5. 1. Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China; Department of Endocrinology, Huai'an Second People's Hospital and the Affiliated Huai'an Hospital of Xuzhou Medical University, Huaian, Jiangsu, China. 2. Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China; Department of Endocrinology, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu, China. 3. Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China. 4. Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China. Electronic address: huyunwuxi@163.com. 5. Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China. Electronic address: maoxming@163.com.
Abstract
AIMS: To evaluate the effect of short-term intensive insulin therapy on circulating T cell subpopulations in patients with newly diagnosed type 2 diabetes mellitus (T2DM). METHODS: A total of 113 patients with T2DM and 28 normal subjects were enrolled. Demographic parameters and biochemical markers were collected at baseline, and flow cytometry was applied to determine the proportion of T cell subpopulations in participants. Then the patients underwent continuous subcutaneous insulin injection (CSII) treatment with euglycemia for 2 weeks, and the T cell subpopulations were measured again after CSII treatment. RESULTS: Compared with normal subjects, the proportion of Th1 cells and the ratio of Th1/Th2 increased, the proportion of Treg cells decreased in patients with T2DM (p < 0.05 for all). The ratio of Th1/Th2 was positively correlated with glycosylated hemoglobin A1c (HbA1c) and negatively correlated with high density lipoprotein cholesterol (HDL-C). Furthermore, there were negative associations between the proportion of Treg cells and fasting plasma glucose, HbA1c, triglyceride, low density lipoprotein cholesterol, and positive association between the proportion of Treg cells and HDL-C. After CSII treatment, the proportion of Th1 cells and the ratio of Th1/Th2 decreased (p < 0.05 for both), the proportion of Treg cells increased in patients with T2DM (p < 0.05). CONCLUSIONS: Short-term intensive insulin therapy could modulate circulating T cell subpopulations in patients with T2DM, which might alleviate inflammatory responses caused by hyperglycemia. This study was registered with ChiCTR-OPN-17010405.
AIMS: To evaluate the effect of short-term intensive insulin therapy on circulating T cell subpopulations in patients with newly diagnosed type 2 diabetes mellitus (T2DM). METHODS: A total of 113 patients with T2DM and 28 normal subjects were enrolled. Demographic parameters and biochemical markers were collected at baseline, and flow cytometry was applied to determine the proportion of T cell subpopulations in participants. Then the patients underwent continuous subcutaneous insulin injection (CSII) treatment with euglycemia for 2 weeks, and the T cell subpopulations were measured again after CSII treatment. RESULTS: Compared with normal subjects, the proportion of Th1 cells and the ratio of Th1/Th2 increased, the proportion of Treg cells decreased in patients with T2DM (p < 0.05 for all). The ratio of Th1/Th2 was positively correlated with glycosylated hemoglobin A1c (HbA1c) and negatively correlated with high density lipoprotein cholesterol (HDL-C). Furthermore, there were negative associations between the proportion of Treg cells and fasting plasma glucose, HbA1c, triglyceride, low density lipoprotein cholesterol, and positive association between the proportion of Treg cells and HDL-C. After CSII treatment, the proportion of Th1 cells and the ratio of Th1/Th2 decreased (p < 0.05 for both), the proportion of Treg cells increased in patients with T2DM (p < 0.05). CONCLUSIONS: Short-term intensive insulin therapy could modulate circulating T cell subpopulations in patients with T2DM, which might alleviate inflammatory responses caused by hyperglycemia. This study was registered with ChiCTR-OPN-17010405.