Luis Apaza T1, Victor Tena Pérez2, Andreea Madalina Serban3, Matías J Alonso Navarro2, Angel Rumbero2. 1. Department of Chemistry, Faculty of Sciences, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain; Department of Pharmacology, Faculty of Pharmacy, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. Electronic address: luis.apaza@uam.es. 2. Department of Chemistry, Faculty of Sciences, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. 3. Marie Schlodowska Curie University Hospital for Children, Constantin Brancoveanu Boulevard, 077120 Bucharest, Romania.
Abstract
ETHNO-PHARMACOLOGICAL RELEVANCE: Tropaeolum tuberosum, commonly known as "Mashua", is one of the plants most frequently used by Andean (Peruvian-Bolivian) people as food and medicine. It is used as a remedy against a wide range of diseases, especially those related with inflammation. OBJECTIVES: This study aims to identify compounds active against inflammatory related conditions. MATERIALS AND METHODS: A bioassay-guided isolation of anti-inflammatory compounds from black and purple tubers of T. tuberosum was performed measuring TNF-α and NF-κB production in THP-1 monocytic cells. RESULTS: The bioassay-guided isolation led to one active compound from purple T. tuberosum, N-oleoyldopamine (1), and another active compound from black T. tuberosum, N-(2-Hydroxyethyl)-7Z,10Z,13Z,16Z-docosatetraenamide (2). Both compounds displayed anti-TNF-α activity with IC50 values of 3.12 ± 0.19 μM and 1.56 ± 0.15 μM, respectively. Also, both compounds suppressed NF-κB with IC50 of 3.54 ± 0.02 μM and 1.77 ± 0.07 μM, respectively. CONCLUSIONS: We identified bioactive compounds from purple and black Tropaeolum tuberosum responsible for their anti-inflammatory activity: N-oleoyldopamine (1) and N-(2-Hydroxyethyl)-7Z,10Z,13Z,16Z-docosatetraenamide (2). This is the first report which isolates these compounds from T. tuberosum and describes their anti-inflammatory activities.
ETHNO-PHARMACOLOGICAL RELEVANCE: Tropaeolum tuberosum, commonly known as "Mashua", is one of the plants most frequently used by Andean (Peruvian-Bolivian) people as food and medicine. It is used as a remedy against a wide range of diseases, especially those related with inflammation. OBJECTIVES: This study aims to identify compounds active against inflammatory related conditions. MATERIALS AND METHODS: A bioassay-guided isolation of anti-inflammatory compounds from black and purple tubers of T. tuberosum was performed measuring TNF-α and NF-κB production in THP-1 monocytic cells. RESULTS: The bioassay-guided isolation led to one active compound from purple T. tuberosum, N-oleoyldopamine (1), and another active compound from black T. tuberosum, N-(2-Hydroxyethyl)-7Z,10Z,13Z,16Z-docosatetraenamide (2). Both compounds displayed anti-TNF-α activity with IC50 values of 3.12 ± 0.19 μM and 1.56 ± 0.15 μM, respectively. Also, both compounds suppressed NF-κB with IC50 of 3.54 ± 0.02 μM and 1.77 ± 0.07 μM, respectively. CONCLUSIONS: We identified bioactive compounds from purple and black Tropaeolum tuberosum responsible for their anti-inflammatory activity: N-oleoyldopamine (1) and N-(2-Hydroxyethyl)-7Z,10Z,13Z,16Z-docosatetraenamide (2). This is the first report which isolates these compounds from T. tuberosum and describes their anti-inflammatory activities.
Authors: Carmen R Silva-Correa; Greysi I Pazo-Medina; Víctor E Villarreal-La Torre; Abhel A Calderón-Peña; Cinthya L Aspajo-Villalaz; José L Cruzado-Razco; Jorge Del Rosario-Chávarri; Anabel D González-Siccha; Luz M Guerrero-Espino; María V González-Blas; William A Sagástegui-Guarniz; César D Gamarra-Sánchez; Julio Hilario-Vargas Journal: Vet World Date: 2022-02-19