Literature DB >> 3074923

Extraction of plant proteins for two-dimensional electrophoresis.

F Granier1.   

Abstract

Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.

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Year:  1988        PMID： 3074923     DOI： 10.1002/elps.1150091106

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  24 in total

1.  Responses of sugar beet roots to iron deficiency. Changes in carbon assimilation and oxygen use.

Authors:  A F López-Millán; F Morales; S Andaluz; Y Gogorcena; A Abadía; J De Las Rivas; J Abadía
Journal:  Plant Physiol       Date:  2000-10       Impact factor: 8.340

2.  Differential expression of leaf proteins in four cultivars of peanut (Arachis hypogaea L.) under water stress.

Authors:  Padmavathi A V Thangella; Srinivas N B S Pasumarti; Raghu Pullakhandam; Bhanuprakash Reddy Geereddy; Manohar Rao Daggu
Journal:  3 Biotech       Date:  2018-03-02       Impact factor: 2.406

3.  Effect of water stress on lupin stem protein analysed by two-dimensional gel electrophoresis.

Authors:  C Pinheiro; J Kehr; C P Ricardo
Journal:  Planta       Date:  2005-01-25       Impact factor: 4.116

4.  Identification and characterization of a developmentally regulated protein, EshA, required for sporogenic hyphal branches in Streptomyces griseus.

Authors:  J Kwak; L A McCue; K Trczianka; K E Kendrick
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

5.  Two dimensional electrophoresis of thylakoid membrane proteins and its application to microsequencing.

Authors:  S G Yu; H Stefansson; E Romanowska; P Å Albertsson
Journal:  Photosynth Res       Date:  1994-09       Impact factor: 3.573

6.  Covalent dimerization of ribulose bisphosphate carboxylase subunits by UV radiation.

Authors:  R M Ferreira; E Franco; A R Teixeira
Journal:  Biochem J       Date:  1996-08-15       Impact factor: 3.857

7.  Alteration of Gene Expression Associated with Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells.

Authors:  Z. Xin; P. H. Li
Journal:  Plant Physiol       Date:  1993-01       Impact factor: 8.340

8.  Comparative Analysis of Short- and Long-Term Changes in Gene Expression Caused by Low Water Potential in Potato (Solanum tuberosum) Cell-Suspension Cultures.

Authors:  A. Leone; A. Costa; M. Tucci; S. Grillo
Journal:  Plant Physiol       Date:  1994-10       Impact factor: 8.340

9.  Comparative proteomics of Cannabis sativa plant tissues.

Authors:  Tri J Raharjo; Ivy Widjaja; Sittiruk Roytrakul; Robert Verpoorte
Journal:  J Biomol Tech       Date:  2004-06

10.  Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers.

Authors:  Lambert C M Ngoka
Journal:  Proteome Sci       Date:  2008-10-24       Impact factor: 2.480
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