Jenelle Izer1, Erin Mattern2, Joy Ellwanger2, Ronald Wilson2. 1. Department of Comparative Medicine, Penn State Milton S Hershey Medical Center, Penn State College of Medicine, Hershey, PA, USA. Electronic address: jizer@pennstatehealth.psu.edu. 2. Department of Comparative Medicine, Penn State Milton S Hershey Medical Center, Penn State College of Medicine, Hershey, PA, USA.
Abstract
OBJECTIVE: To determine the degree of agreement between arterial and venous blood gases in anesthetized lambs using a point-of-care analyzer. STUDY DESIGN: Prospective experimental study. ANIMALS: A group of 12 female Dorset cross-bred lambs, weighing 37.3 ± 7.4 kg (mean ± standard deviation). METHODS: Lambs were anesthetized with isoflurane for catheterization of the jugular vein and femoral artery, and blood samples were collected simultaneously for analysis of pH, PCO2, PO2, base excess (BE), bicarbonate ( [Formula: see text] ), total carbon dioxide (tCO2), oxygen saturation (SO2), ionized calcium (iCa) and potassium (K+) using the Vet Scan i-STAT handheld analyzer. The Bland-Altman method was used to calculate agreement between arterial and venous measurements. RESULTS: Strong agreement was identified between arterial and venous pH (bias = 0.04; 95% limits of agreement = 0.02-0.06), K+ (bias = -0.1 and 95% limits of agreement = -0.8 to 0.5) and iCa (bias = 0.04; 95% limits of agreement = -0.08 to 0.0003). There was poor agreement for PO2 (bias = 168.0; 95% limits of agreement = 77.4-258.7) and SO2 (bias = 9.1; 95% limits of agreement = 2.8-15.3), as these values were higher in arterial blood than in venous blood. Moderate agreement was present for BE (bias = 1.7; 95% limits of agreement = -2.7 to 6.1), PCO2 (bias = -2.1; 95% limits of agreement = -7.5 to 3.4), [Formula: see text] (bias = 1.1; 95% limits of agreement = -3.1 to 5.3) and tCO2 (bias = 1.0; 95% limits of agreement = -3.3 to 5.3). CONCLUSIONS AND CLINICAL RELEVANCE: Venous blood can be used for the measurement of pH, K+ and iCa in anesthetized Dorset cross-bred lambs. Arterial blood is required for accurate measurement of PO2, SO2, PCO2, [Formula: see text] , tCO2 and BE to assess systemic blood oxygenation and pulmonary function in anesthetized Dorset cross-bred lambs.
OBJECTIVE: To determine the degree of agreement between arterial and venous blood gases in anesthetized lambs using a point-of-care analyzer. STUDY DESIGN: Prospective experimental study. ANIMALS: A group of 12 female Dorset cross-bred lambs, weighing 37.3 ± 7.4 kg (mean ± standard deviation). METHODS:Lambs were anesthetized with isoflurane for catheterization of the jugular vein and femoral artery, and blood samples were collected simultaneously for analysis of pH, PCO2, PO2, base excess (BE), bicarbonate ( [Formula: see text] ), total carbon dioxide (tCO2), oxygen saturation (SO2), ionized calcium (iCa) and potassium (K+) using the Vet Scan i-STAT handheld analyzer. The Bland-Altman method was used to calculate agreement between arterial and venous measurements. RESULTS: Strong agreement was identified between arterial and venous pH (bias = 0.04; 95% limits of agreement = 0.02-0.06), K+ (bias = -0.1 and 95% limits of agreement = -0.8 to 0.5) and iCa (bias = 0.04; 95% limits of agreement = -0.08 to 0.0003). There was poor agreement for PO2 (bias = 168.0; 95% limits of agreement = 77.4-258.7) and SO2 (bias = 9.1; 95% limits of agreement = 2.8-15.3), as these values were higher in arterial blood than in venous blood. Moderate agreement was present for BE (bias = 1.7; 95% limits of agreement = -2.7 to 6.1), PCO2 (bias = -2.1; 95% limits of agreement = -7.5 to 3.4), [Formula: see text] (bias = 1.1; 95% limits of agreement = -3.1 to 5.3) and tCO2 (bias = 1.0; 95% limits of agreement = -3.3 to 5.3). CONCLUSIONS AND CLINICAL RELEVANCE: Venous blood can be used for the measurement of pH, K+ and iCa in anesthetized Dorset cross-bred lambs. Arterial blood is required for accurate measurement of PO2, SO2, PCO2, [Formula: see text] , tCO2 and BE to assess systemic blood oxygenation and pulmonary function in anesthetized Dorset cross-bred lambs.
Authors: Lin Xu; Ashok Chockalingam; Sharron Stewart; Katherine Shea; Murali K Matta; Suresh Narayanasamy; Nageswara R Pilli; Donna A Volpe; James Weaver; Hao Zhu; Michael C Davis; Rodney Rouse Journal: Toxicol Rep Date: 2020-01-25