Phytotoxic effects of silver nanoparticles and silver ions to Arabidopsis thaliana as revealed by analysis of molecular responses and of metabolic pathways.

The acute (3 days) and chronic (whole life history) responses of Arabidopsis thaliana following exposure to silver nanoparticles (AgNPs) and Ag+ ions (AgNO3) in respectively a hydroponic medium and in soil were studied. After 3 days of hydroponic exposure, AgNPs (1.0 and 2.5 mg/L) exerted more severe inhibitory effects on plant (shoot and root) growth and photosynthesis than the same concentrations of Ag+ ions. In soil cultivation, the photoperiod, the autonomous, and the vernalization pathways were down-regulated to 0.15- to 0.5-fold of the control after 12.5 mg/kg AgNPs treatment. This exposure caused a decrease of approximately 25%-40% as compared to the control of the transcription of flowering key genes including AP1, LFY, FT and SOC1, and finally resulted in a delayed flowering time of 5 days. Only autonomous and vernalization pathways were inhibited by Ag+ ion treatment and ultimately the time of flowering in treated plants was delayed by 3 days. The energy production related metabolic pathways in the tricarboxylic acid cycle and in sugar metabolism were stimulated stronger by AgNPs than by Ag+ ion treatment, thus releasing more energy and accelerating the physiological metabolic responses against stress in the AgNPs treatment while subsequently reducing the plant growth and yield at the maturation stage. Importantly, shikimate-phenylpropanoid biosynthesis, and tryptophan and galactose metabolisms were regulated only by the AgNPs treatment, which was a specific effect of nanoparticles. This work provides a systematic understanding at the molecular, physiological as well as metabolic level of the effects of AgNPs and Ag+ ions in A. thaliana.