Eve Todesco1, Nesrine Day2, Corinne Amiel3, Stéphane Elaerts2, Véronique Schneider3, Laurent Roudière4, Stéphane Hué5, Jean-Yves Liotier6, Julie Bottero7, Thomas L'Yavanc8, Michel Ohayon8, Daniel Gosset8, Vincent Thibault9, Laure Surgers10, Julie Chas11, Sepideh Akhavan12, Annie Velter13, Christine Katlama14, Georges Kreplak2, Anne-Geneviève Marcelin15, Marc-Antoine Valantin14. 1. Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, Paris, France. Electronic address: eve.todesco@aphp.fr. 2. Cerballiance Laboratory, Paris, France. 3. Sorbonne Université, Centre d'Immunologie et de Maladies Infectieuses UMRS CR7, Persistent Viral Infection Team, Inserm U1135, APHP, Groupe Hospitalier Paris Est, Hôpital Tenon, Laboratoire de virologie, Paris, France. 4. AP-HP, Hôpital Pitié-Salpêtrière, Department of Internal Medicine, Paris, France. 5. Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK. 6. APHP, Hôpital Bicêtre, Department of Infectious Diseases, Le Kremlin-Bicêtre, France. 7. Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpital Saint Antoine, Department of Infectious Diseases, Paris, France. 8. Centre de santé sexuelle Le 190, Paris, France; Sorbonne Université, APHP, Hôpital Tenon, Department of Infectious Diseases, Paris, France. 9. Department of Virology, CHU de Rennes, Univ Rennes INSERM IRSET UMR_S 1085, Université Rennes 1, Rennes, France. 10. Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpital Saint Antoine, Department of Infectious Diseases, Paris, France; Sorbonne Université, INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris, France. 11. Sorbonne Université, APHP, Hôpital Tenon, Department of Infectious Diseases, Paris, France. 12. Sorbonne Université, INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Persistent Viral Infection Team, AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, Paris, France. 13. Santé publique France, Saint-Maurice, France. 14. Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpital Pitié-Salpêtrière, Department of Infectious Diseases, Paris, France. 15. Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, Paris, France.
Abstract
BACKGROUND: Increasing incidence of hepatitis C virus (HCV) infection in human immunodeficiency virus (HIV)-positive men having sex with men (MSM) has been described in recent years. Phylogenetic analyses of acute HCV infections were undertaken to characterize the dynamics during the epidemic in Paris, and associated sexually transmitted infections (STIs) were evaluated. METHODS: Sanger sequencing of polymerase gene was performed. Maximum likelihood phylogenies were reconstructed using FastTree 2.1 under a GTR+CAT model. Transmission chains were defined as clades with a branch probability ≥0.80 and intraclade genetic distances <0.02 nucleotide substitutions per sites. STIs detected ≤1 month before HCV diagnosis were considered. RESULTS: Among the 85 studied patients, at least 81.2% were MSM. Respectively, 47.6%, 39.0%, 11.0% and 2.4% were infected with genotypes 1a, 4d, 3a and 2k. At least 91.8% were co-infected with HIV. HCV re-infection was evidenced for 24.7% of patients and STIs for 20.0% of patients. Twenty-two transmission chains were identified, including 52 acute hepatitis C (11 pairs and 11 clusters from three to seven patients). CONCLUSIONS: These results revealed strong clustering of acute HCV infections. Thus, rapid treatment of both chronic and acute infections is needed among this population to decrease the prevalence of HCV, in combination with preventive behavioural interventions.
BACKGROUND: Increasing incidence of hepatitis C virus (HCV) infection in human immunodeficiency virus (HIV)-positive men having sex with men (MSM) has been described in recent years. Phylogenetic analyses of acute HCV infections were undertaken to characterize the dynamics during the epidemic in Paris, and associated sexually transmitted infections (STIs) were evaluated. METHODS: Sanger sequencing of polymerase gene was performed. Maximum likelihood phylogenies were reconstructed using FastTree 2.1 under a GTR+CAT model. Transmission chains were defined as clades with a branch probability ≥0.80 and intraclade genetic distances <0.02 nucleotide substitutions per sites. STIs detected ≤1 month before HCV diagnosis were considered. RESULTS: Among the 85 studied patients, at least 81.2% were MSM. Respectively, 47.6%, 39.0%, 11.0% and 2.4% were infected with genotypes 1a, 4d, 3a and 2k. At least 91.8% were co-infected with HIV. HCV re-infection was evidenced for 24.7% of patients and STIs for 20.0% of patients. Twenty-two transmission chains were identified, including 52 acute hepatitis C (11 pairs and 11 clusters from three to seven patients). CONCLUSIONS: These results revealed strong clustering of acute HCV infections. Thus, rapid treatment of both chronic and acute infections is needed among this population to decrease the prevalence of HCV, in combination with preventive behavioural interventions.
Authors: Sofia R Bartlett; Andrey Verich; Joanne Carson; Samira Hosseini-Hooshyar; Phillip Read; David Baker; Jeffrey J Post; Robert Finlayson; Mark Bloch; Joseph S Doyle; David Shaw; Margaret Hellard; Maria Martinez; Philippa Marks; Gregory J Dore; Gail V Matthews; Tanya Applegate; Marianne Martinello Journal: Health Sci Rep Date: 2022-08-18
Authors: David Chromy; David J M Bauer; Benedikt Simbrunner; Matthias Jachs; Lukas Hartl; Philipp Schwabl; Caroline Schwarz; Armin Rieger; Katharina Grabmeier-Pfistershammer; Michael Trauner; Peter Ferenci; Mattias Mandorfer; Michael Gschwantler; Thomas Reiberger Journal: J Viral Hepat Date: 2022-03-15 Impact factor: 3.517