Hanae Naceiri Mrabti1, Moulay El Abbes Faouzi1, François Massako Mayuk2, Hanane Makrane3, Nicolas Limas-Nzouzi3, Siegfried Didier Dibong4, Yahia Cherrah1, Ferdinand Kouoh Elombo3, Bernard Gressier5, Jehan-François Desjeux6, Bruno Eto7. 1. Laboratory of Pharmacology and Toxicology, Research Team of Bio Pharmaceutical and Toxicological Analyzes, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat BP 6203, Morocco. 2. Etobiotech Cameroun and Laboratory of Biology and Physiology of Plant Organisms, Department of Plant Biology, University of Douala, Cameroon; Laboratoires TBC, Faculty of Pharmaceutical and Biological Sciences, Lille, France. 3. Laboratoires TBC, Faculty of Pharmaceutical and Biological Sciences, Lille, France. 4. Etobiotech Cameroun and Laboratory of Biology and Physiology of Plant Organisms, Department of Plant Biology, University of Douala, Cameroon. 5. Laboratory of Pharmacology, Pharmacokinetics and Clinical Pharmacy, Faculty of Pharmaceutical and Biological Sciences, Lille, France. 6. Académie Nationale de Médecine, Paris, France. 7. Laboratoires TBC, Faculty of Pharmaceutical and Biological Sciences, Lille, France. Electronic address: etobr@laboratoires-tbc.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Arbutus unedo L., (Ericaceae) is one of the most traditional plants commonly used to treat diabetes in people living in Eastern Morocco region particularly in Taza and Beni Mellal. AIM OF THE STUDY: The aim of the study was to find if there is a scientific support to the ethnopharmacological relevance use of Arbutus unedo L., roots bark (AU) to treat diabetes. MATERIALS AND METHODS: We studied the effects of crude aqueous extract of AU on intestinal glucose absorption using short-circuit current technique in vitro and oral glucose tolerance test in vivo. RESULTS: The aqueous extract of AU (10 µg/mL to 1 mg/mL) induced concentration-dependent inhibition of sodium-dependent glucose transport across isolated mouse jejunum. The maximal inhibition was obtained with 1 mg/mL, which exhibited more than 80% of the Phloridzin inhibition with an IC50 close to 216 µg/mL. A 6-week AU ingestion (2 g/(kg day)), improved oral glucose tolerance as efficiently as metformin (300 mg/(kg day)). Arbutus unedo L. and metformin also reduced body weight. CONCLUSIONS: Arbutus unedo L. roots bark aqueous extract directly inhibited the electrogenic intestinal absorption of glucose in vitro. In addition it improved oral glucose tolerance and lowered body weight in rats after chronic oral administration in vivo. These results add a scientific support to the ethnopharmacological relevance use of Arbutus unedo L. roots bark to treat diabetes.
ETHNOPHARMACOLOGICAL RELEVANCE: Arbutus unedo L., (Ericaceae) is one of the most traditional plants commonly used to treat diabetes in people living in Eastern Morocco region particularly in Taza and Beni Mellal. AIM OF THE STUDY: The aim of the study was to find if there is a scientific support to the ethnopharmacological relevance use of Arbutus unedo L., roots bark (AU) to treat diabetes. MATERIALS AND METHODS: We studied the effects of crude aqueous extract of AU on intestinal glucose absorption using short-circuit current technique in vitro and oral glucose tolerance test in vivo. RESULTS: The aqueous extract of AU (10 µg/mL to 1 mg/mL) induced concentration-dependent inhibition of sodium-dependent glucose transport across isolated mouse jejunum. The maximal inhibition was obtained with 1 mg/mL, which exhibited more than 80% of the Phloridzin inhibition with an IC50 close to 216 µg/mL. A 6-week AU ingestion (2 g/(kg day)), improved oral glucose tolerance as efficiently as metformin (300 mg/(kg day)). Arbutus unedo L. and metformin also reduced body weight. CONCLUSIONS:Arbutus unedo L. roots bark aqueous extract directly inhibited the electrogenic intestinal absorption of glucose in vitro. In addition it improved oral glucose tolerance and lowered body weight in rats after chronic oral administration in vivo. These results add a scientific support to the ethnopharmacological relevance use of Arbutus unedo L. roots bark to treat diabetes.
Authors: Maria Concetta Tenuta; Brigitte Deguin; Monica Rosa Loizzo; Annabelle Dugay; Rosaria Acquaviva; Giuseppe Antonio Malfa; Marco Bonesi; Chouaha Bouzidi; Rosa Tundis Journal: Antioxidants (Basel) Date: 2020-02-22
Authors: Salima Boutahiri; Mohamed Bouhrim; Chayma Abidi; Hamza Mechchate; Ali S Alqahtani; Omar M Noman; Ferdinand Kouoh Elombo; Bernard Gressier; Sevser Sahpaz; Mohamed Bnouham; Jehan-François Desjeux; Touriya Zair; Bruno Eto Journal: Pharmaceutics Date: 2021-11-26 Impact factor: 6.321