| Literature DB >> 30742272 |
Theresa Chow1,2, Jennifer Whiteley1, Ian M Rogers3,4,5.
Abstract
Decellularization is the process by which resident cells are lysed and cellular debris is removed from the tissue, leaving behind the extracellular matrix scaffold. The extracellular matrix scaffold can be used for three-dimensional culturing of cells. Here we describe methods of decellularizing whole and thick sections of mouse kidneys using a 0.1% sodium dodecyl sulfate (SDS) detergent solution and strategies to repopulate whole and thick sections of decellularized mouse kidneys with cells.Entities:
Keywords: Bioreactor; Cannulation and perfusion; Extracellular matrix; Kidney decellularization; Kidney recellularization; Mouse kidney dissection; Three-dimensional (3D) tissue culture
Mesh：
Year: 2019 PMID： 30742272 DOI： 10.1007/978-1-4939-9021-4_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745